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Vol.4 No.1 – 1 : Histological and histochemical changes in liver of gamma-irradiated rats and the possible protective role of Aphanizomenon flos-aquae (AFA)

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By : Hemmat Mansour Abdelhafez and Heba Ahmed Mohamed Kandeal

Abstract

Exposure to ionizing radiation represents a genuine increasing threat to mankind and our environment. Aphanizomenon flos-aquae (AFA) is a blue-green microalgal species which has antioxidant properties. The Aim of the work: this study aimed to elucidate the possible radioprotective effect of Aphanizomenon flos-aquae (AFA) on liver of irradiated adult male rats using biochemical parameters, histopathology and quantitative histochemistry. Matrerial and methods: the current experiment was carried out on 48 adult male albino rats (Rattus rattus). Rats were randomly and equally categorized into four groups: 1) Group C: control rats left without treatment; 2) Group R: rats were exposed to 4Gy of gamma-radiation as a single dose; 3) Group AFA: rats were treated orally with 94.5mg/kg body weight/ day AFA for 3 weeks and 4) Group AFA+R: rats were administrated AFA for a period of one week before and three weeks after irradiation. The experimental rats were sacrificed after 5 and 21 days post-irradiation. Results: exposed to gamma radiation showed many biochemical changes which included a significant increase in serum ALAT, ASAT ,ALP activities and MDA in the liver tissues . Many histopathological and histochemical changes were observed in the liver tissue, such as corrugated and ruptured endothelial lining of the central vein which contained hemolysed blood cells, numerous vacuolated hepatocytes with increased signs of karyolysis and pyknosis in nuclei of hepatocytes, highly dilated and congested hepatic portal vein, numerous hemorrhagic areas and distorted bile ducts. Highly increased collagen fibers were also observed after gamma irradiation in the liver tissue. In addition, irradiated group induced a significant increase in amyloid β-protein, while a significant decrease in PAS+ve materials, total protein  and total DNA content was detected. Supplementation with AFA showed a trend toward lowering incidence of hepatic histopathological and histochemical changes induced by γ-radiation.

Conclusion: according to the results obtained in the current study using Aphanizomenon flos- aquae as a natural agent showed a strong radioprotective role.

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Vol.2 No.6 -10 : The radioprotective role of Aphanizomenon flos-aquae (AFA) on testis of adult male albino rats

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By : Fatma Ahmed Eid , Hemmat Mansour Abdelhafez ,Samir Attia Zahkouk1 and Heba Ahmed

Mohamed Kandeal

Abstract

This work aimed to study the biochemical, histopathological and histochemical changes in the testes of
male albino rats post exposure to 4 Gy of gamma radiation and the possible radioprotective role of Aphanizomenon flos-aquae (AFA). Aphanizomenon flos-aquae (AFA) is a blue-green microalgal species which has antioxidant properties. The current experiment was carried out on 48 adult male albino rats (Rattus rattus). Rats were randomly and equally categorized into four groups: 1) Group C: control rats left without treatment; 2) Group R: rats were exposed to 4Gy of gamma-radiation as a single dose; 3)
Group AFA: rats were terated orally with 94.5mg/kg body weight/ day AFA for 3 weeks and 4) Group AFA+R: rats were administrated AFA for a period of one week before and three weeks after irradiation. Results obtained in the present study showed that exposed rats showed a significant increase in MDA in the testes, but decreased testosterone level was detected versus the control. Many histopathological lesions were observed in the testes tissue such as disturbed spermatogenic layers with vacuolated spermatogenic cells, presence of polynucleated cells, absence of mature sperms, oedema in the interstitial spaces, congested testicular arteries with thickened, dilated and corrugated walls of them, they also contained hemolysed blood cells with highly reduced, atrophied and distorted Leydig cells in the interstitial spaces. Irradiated groups showed highly increased collagen fibres under the testicular capsule, basement membranes, Leydig cells and around the blood vessels with signs of fibrosis in the capsule and some seminiferous tubules In addition, irradiated group induced a significant increase in amyloid - protein, while a significant decrease in PAS+ve materials, total protein and total DNA content was detected. AFA administration ameliorated the damaging effects of testes of radiation exposed rats. Conclusion: according to the results obtained in the current study using Aphanizomenon flos- aquae as a natural agent showed a strong radioprotective role. Key words. Gamma rays, ionizing radiation, Aphanizomenon flos-aquae (AFA), testes, rats.

Vol.2-No.6-10-The-radioprotective-role-of-Aphanizomenon-flos-aquae-AFA-on-testis-of-adult-male-albino-rats

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