By : Mohamed A. Ismail

Abstract

Acrylamide (AA) is formed in food during heat preparation (frying and baking), causing DNA toxicity. So, the aim of this study is applied β-1,3-D-Glucan (BDG) as a natural polysaccharide ameliorative to reduce the DNA hepatotoxicity and genotoxicity of bone-marrow chromosomes in male mice by using three parameters: alkaline comet assay, cytochemical DNA and cytogenetical protocols. The AA-oral fed mice are classified into three groups, the first received low dose and the second intake the double fashion of AA in alone or concomitant with BDG for 30 days, besides the fourth group of controls. The study observed that AA induced both numerical and structural chromosomal aberrations in a significant increase (p<0.05 or p<0.0001) in a dose-dependent relationship. The cytochemical study on DNA exhibited that the AA-treated hepatocyte nuclei, showed strong stainability with condensed DNA inclusions and releasing outside their nuclear envelops. Under the comet assay conditions, AA-treated hepatocytes revealed a distinct comet tail electrophoretic migration of DNA fragments that resulted from AA-induced DNA strand breaks. The study also observed similarity configurations of AA-DNA fragmented damage between the findings of cytochemical DNA and comet assays in hepatocytes, and reinforced with the stretching and pulverized chromosome aberrations. But, after enhancing with BDG, the most implications of AA were inclined into mitigation as detected by microscopical and by 3D-comet image analysis, to indicate the potential alleviation role of BDG on AA-induced DNA alterations in hepatocytes and chromosomes of mice.

8_Ameliorative_potential_of_β-1_3-D-Glucan_on_acrylamide-induced_cytogenetic_alterations_in_mice
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