Vol.1 No.6 -11 : Histological and Biological Effects of Some Soft Drinks on Male Albino Rats

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By : Nehad R. EL-Tahan1 and Rania A. Ahmed2

Abstract

sodas that could have a caloric or noncaloric sweetener. Several studies showed that the excessive consumption of soft drinks leads to harmful health effects. This study aimed to determine the effects of some soft drinks on the public health by examining the histological structure of kidney, body weight, biochemical values, bone mineral density (BMD ) and bone Xray . Twenty male rats, (weighing 140 ±5 g) , were divided into 4 groups. ; Group 1 (the control group) fed only on basal diet. ; Group 2 received cola diet soft drink ; Group 3 received orange soft drink and Group 4 received lemon soda soft drink. For histopathological examination, The kidneys were removed. The bone mineral density of the rats was assessed using Dual-energy X-ray absorptiometry (DXA). The body weight change and blood values were also determined. The histological examination of the kidneys showed general glomerular congestion, vacuolation, intertubular bleeding, tubular necrosis and hypotrophy of glomeruli . The weight gain was higher in the groups consuming diet drinks than the control group and the other group rats. There was a significant decrease in the bone mineral density of test groups when compared to the control group .

11. Histological and Biological Effects of Some Soft Drinks on Male Albino Rats

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Vol.1 No.6 – 12: Evaluation of Nuclepore Membrane Filtration Technique for Diagnosis of Schistosoma haematobium Infection

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By : Ahmed Abdelhalim Yameny

Society of Pathological Biochemistry and Hematology, Egypt

Ahmed A. Yameny (Email: dr.ahmedyameny@yahoo.com)

Abstract

Schistosomiasis is a parasitic disease. It is one of the most important diseases of humans in tropical and subtropical parts of the world, rapid characterization of schistosome eggs considered a key step for clinical management, diagnosis of Schistosoma haematobium is generally based on the detection of eggs in a single urine specimen, Urine filtration is one of the methods recommended by the WHO for the detection of S.haematobium. In 1976, Peters et al adapted a nuclepore filter for the diagnosis of S.haematobium eggs. They used a filter 13 mm in diameter with 8 µm pores, in another study done by Peters et al (1976), they proved that under field conditions, filtration of two 5 ml aliquots using 8 µm pore filters was very practical, if eggs of S.haematobium are present (size 150 by 60 µm), they are unable to pass through the filter and can be observed and counted under a microscope fitted with a 10x objective.

This study used both the microscopic examination techniques of urine, centrifugation sedimentation technique and Nuclepore membrane filtration technique to detect Schistosoma haematobium eggs in urine for 1000 patient samples, to evaluate each technique alone and the two techniques together. This study showed that the nuclepore filtration method more valid than the sedimentation centrifugation method, nuclepore filtration detect 77 infected cases with a sensitivity of 97.5%, but sedimentation detect 64 infected cases with a sensitivity of 81%, in this study we considered the sum of nuclepore and sedimentation as a gold standard, they detected 79 infected cases to compare them with other methods.

Evaluation-of-Nuclepore-Membrane-Filteration-Technique-for-Diagnosis-of-Schistosoma-haematobium-Infection-Final-converted

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