Vol.6 No.1 – 1: A pilot study of the paraoxonase-1 (Q192 R) gene polymorphism association with prostate cancer in the Egyptian population

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By: Hany Abd Al Hamid1, Mohamed Y. Nasr1, Asmaa Ibrahim1,3, Khalid Bassiouny1,  Manal Mohamed Abd Al Aziz2

1. Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), Egypt

2. Clinical Pathology Department, Faculty of Medicine, Ain shams University, Cairo, Egypt

3.Diagnostic and Research Unit of Parasitic Diseases (DRUP), Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Background and Objectives: Prostate cancer (PCa) is one of the most common cancer types in men and recognized as the fifth cause of death globally. Human paraoxanase-1 (PON1) is an enzyme synthesized in the liver and linked with high-density lipoprotein (HDL). The current study aimed to evaluate the association between PCa and PON1 enzyme polymorphism in Egyptian patients.

Subjects and methods: A total of 100 persons. Fifty patients diagnosed as PCa patients (group I) along with fifty non-PCa (group II) of matchable age were enrolled in this study. Patients with another type of cancer or any chronic disease were excluded. Prostate-specific antigen (PSA) was assayed in all subject’s study by using ELISA Kit Protocol (Cat. No.: EK-310-19); in addition to CBC, hematological parameters (hemoglobin, total leucocyte count and platelets count) were estimated by using automated hematology analyzer (Pentra 80) according to manufacturer’s instructions. PON1 Q192R gene polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results: PON1 (Q192R) gene polymorphism showed QR genotype in PCa patients (group I) ]32(64%)[ compared with none-PCa (group II)] 4(8%)[. The association between PON1 (Q192R) gene polymorphism with prostate cancer group and PSA were statistically significant (P value= 0.000). Hemoglobin showed significant association with PCa patients (group I) and PSA (P = 0.000), while total leucocyte count and platelets count showed non-significant when compared with the control groupConclusion: Such findings showed that PON1 (Q192R) gene polymorphism could be associated with prostate cancer and disease growth. Recommendation, furthermore comprehensive stud­ies are required to confirm the role of PON1 gene polymorphism in the etiology of PCa.

A pilot study of the paraoxonase-1 (Q192 R) gene polymorphism association with prostate cancer in Egyptian population-converted

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Vol.5 No.2 – 6 : Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia and their HBV Infection Status

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By: Abdirauf Mohamed Abdi and Mohd Nazil Salleh

Department of Health Sciences, Faculty of Engineering and Life Sciences, Universiti Selangor, Shah Alam City Campus, Jalan Zikron A 7/A, Seksyen 7, 40000 Shah Alam Selangor, Malaysia

Abstract

Background: Hepatitis B virus (HBV) infection is a serious health problem that can lead to liver cirrhosis and cancer. Assessing the public knowledge, attitude and practice towards HBV infection can be useful in planning public health policies in HBV management. Objective: The current study sought to determine the association between knowledge, attitude, and practice towards HBV infection with the HBV infection status of Somalian immigrants in Malaysia. Method: A cross-sectional study was conducted from September 2017 to April 2018 among Somalian immigrants in the state of Selangor, Malaysia. Data was collected using validated, self-administered structured questionnaire. Their blood samples was collected for detection of HBV DNA using nested PCR. Descriptive statistics and chi square analysis were done to determine this association. Result: A total of 145 participants were recruited. Majority of participants were in the age group of 20-31 (84.1%), male (62.8%), single (52.4%), had educational level of undergraduate degree (70.3%), and unemployed (88.3%). Majority of the participants show good knowledge (82.8%) and attitude (78.6%) but generally poor practice (32.4%) towards HBV infection. The mean knowledge, attitude and practice score among them were 16.9 ± (4.89), 9.20 ± (2.94) and 9.39 ± (2.93) respectively. The sociodemographic characteristic variables and KAP of HBV were not significantly associated. Selected samples collected from the participant tested negative for HBV DNA via nested PCR assay. Conclusion: Somalian immigrants in Malaysia have a good level of knowledge and attitude but poor level of practice towards HBV infection. All participants were negative for HBV infection.

Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia and their HBV Infection Status

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Vol.5 No.1 – 4 : Evaluation of the in vitro antiplasmodial activity of Millettia zechiana and its action on the evolution of anemia in albino rats.

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By : Karamoko Chérif Moustapha1,2*, Touré André Offianan2, Bla Kouakou Brice1, Tuo Karim2, N’guessan Yao Honoré1, Bidie Alain dit Philippe1

1University of Félix Houphouët Boigny, 22 PO Box 582 Abidjan 22, Côte d’Ivoire

2 Institut Pasteur of Côte d’Ivoire, 01 PO Box 490 Abidjan 01

Abstract

Background: Malaria is a parasitic infection that leads to anemia and death. Unfortunately, the upsurge of chemo-resistance prompted researchers to focus on new antimalarial drugs. Objectives: This work aimed to evaluate the antiplasmodial and antianemic activity of Millettia zechiana. Methods: the In vitro activity was assessed on clinical isolates and the standard strain of Plasmodium falciparum K1, using the SYBR green I test. Moreover, the antianemic activity was evaluated in phenylhydrazine-induced anemic albino rats. Results/discussion: The ethyl acetate and hydroethanolic extract exhibited an antiplasmodial activity with IC50s of 6.14 and 12.14 μg/mL respectively on the Plasmodium falciparum K1 strain. As for the in vivo antianemic activity, the ethyl acetate extract was the most active with better hematological reconstitution percentages. The presence of chemical compounds such as alkaloids, terpenoids, and quinonic substances in both extracts, could be responsible for their activities. Conclusion: Millettia zechiana could be a potential source for novel antimalarial drugs and might be used as an improved traditional medicine on account of its availability.

Evaluation of the in vitro antiplasmodial activity of Millettia zechiana and its action on the evolution of anemia in albino rats.-converted

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