Vol.6 No.4 – 8:The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients: An Experience from Egypt

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DOI: 10.21608/jbaar.2020.135612

By: Samar Samir Youssef1*, Eman Abd El Razek Abbas1, Yasser Hamada Aly2, Sameh Seif3

  1. of Microbial Biotechnology, National Research Centre, Dokki, Egypt.
  2. Department of Endemic Medicine, Cairo University.
  3. National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt

Abstract

Objectives: To detect the correlation between the single nucleotide polymorphisms (SNPs) in MBOAT7 and PNPLA3 genes and hepatic fibrosis in hepatitis C virus (HCV) Egyptian patients, and to highlight the additive effect, if any, of MBOAT7 on the correlation of PNPLA3 polymorphism with liver fibrosis in HCV patients from Egypt. Methods: In this cross-sectional study, we recruited treatment-naïve patients with chronic HCV. The rs738409 (PNPLA3) and rs641738 (MBOAT7) polymorphisms were assessed by Real-Time PCR. We utilized the METAVIR‐Score to classify the degree of hepatic fibrosis and necroinflammatory activity.

Results: A total of 93 patients (mean age 42.72 ± 10.46; males = 49.5%) were included. Our analysis showed that 10.8% of the patients had GG genotype of the PNPLA3 gene and 46.2% had TT genotype of the MBOAT7 gene.  Compared to combined CC and GC genotypes, carriers of GG genotype in the PNPLA3 gene were more likely to be males (p =0.041), have higher fibrosis grade (p =0.043), have higher serum creatinine (p=0.036), higher TSH (p =0.017) and higher viral load (p =0.045). Notably, we found a significant association between TT genotype in MBOAT7 and advanced fibrosis only (but not with necroinflammation (p >0.05). Our multivariate analysis showed that the GG genotype in the PNPLA3 gene and TT genotype in the MBOAT7 gene were independent predictors of advanced fibrosis. Conclusion: PNPLA3 GG genotype and MBOAT7 TT genotype are independent predictors for hepatic fibrosis, and thus might be linked to faster disease progression.

The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients-converted

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