Vol.10 No.1 – 8: Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections

Noor Ahmad ¹*, Qudus Wamidh ², Thanaa Rasheed Abdulrahman²

Department of Microbiology, Al Nahrain University/ College of Medicine – Iraq

1 Department Microbiology, Medical College/Al Nahrain University, Iraq

2 PhD in Department Microbiology, Medical College/Al Nahrain University, Iraq 

ABSTRACT

Background: Urinary tract infections (UTIs) frequently affect the urinary system, predominantly caused by Escherichia coli (E. coli) and Klebsiella pneumoniae. This study examines the prevalence of these bacteria and bla OXA-48 and bla IMP genes.

Objectives: The research aims to assess the occurrence of MDR and XDR E. coli and K. pneumoniae in pediatric UTI cases, including the resistance genes bla OXA-48 and bla IMP.

Methods: A total of 100 pediatric UTI urine samples were analyzed for antibiotic resistance, ESBL detection, and bla OXA-48 and bla IMP gene presence using molecular techniques, gene sequencing, and statistical analysis via SPSS software.

Results: High resistance rates to common antibiotics were found in K. pneumoniae and E. coli isolates. bla OXA-48 was detected in 92.3% of E. coli and 100% of K. pneumoniae isolates, while bla IMP was found in 43.1% of E. coli and 25.7% of K. pneumoniae isolates. No nucleic acid variation was observed in the blaIMP-1 sequences of both bacteria.

Conclusion: The significant presence of bla OXA-48 and bla IMP genes in K. pneumoniae and E. coli highlights the urgent need for enhanced surveillance and careful antibiotic use to curb antibiotic-resistant UTIs in children.

Download PDF