Vol.10 No.1 – 8: Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections

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Noor Ahmad 1*, Qudus Wamidh 2, Thanaa Rasheed Abdulrahman2

Department of Microbiology, Al Nahrain University/ College of Medicine – Iraq

1 Department Microbiology, Medical College/Al Nahrain University, Iraq

2 PhD in Department Microbiology, Medical College/Al Nahrain University, Iraq 

ABSTRACT

Background: Urinary tract infections (UTIs) frequently affect the urinary system, predominantly caused by Escherichia coli (E. coli) and Klebsiella pneumoniae. This study examines the prevalence of these bacteria and bla OXA-48 and bla IMP genes.

Objectives: The research aims to assess the occurrence of MDR and XDR E. coli and K. pneumoniae in pediatric UTI cases, including the resistance genes bla OXA-48 and bla IMP.

Methods: A total of 100 pediatric UTI urine samples were analyzed for antibiotic resistance, ESBL detection, and bla OXA-48 and bla IMP gene presence using molecular techniques, gene sequencing, and statistical analysis via SPSS software.

Results: High resistance rates to common antibiotics were found in K. pneumoniae and E. coli isolates. bla OXA-48 was detected in 92.3% of E. coli and 100% of K. pneumoniae isolates, while bla IMP was found in 43.1% of E. coli and 25.7% of K. pneumoniae isolates. No nucleic acid variation was observed in the blaIMP-1 sequences of both bacteria.

Conclusion: The significant presence of bla OXA-48 and bla IMP genes in K. pneumoniae and E. coli highlights the urgent need for enhanced surveillance and careful antibiotic use to curb antibiotic-resistant UTIs in children.

Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections

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Vol.10 No.1 – 7: A Prospective study: Correlation between Serum Estradiol levels and Fertilization Rates in Assisted Reproductive Technology Outcomes

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Namir I. A. Haddad 1, Ekhlas Ali Hussein2, Israa Talib Hassan 3

  1. Professor. Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq
  2. FICOG. Professor, Department of Gynecology and Obstetrics, College of Medicine, Al-Iraqia University, IRAQ
  3. FICOG. Professor, Department of Gynecology and Obstetrics, College of Medicine, Al-Iraqia University, IRAQ.

ABSTRACT

Background and Objective: Advancements in reproductive procedures require an understanding of factors influencing in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) outcomes. The study objective is to evaluate the impact of serum estradiol (E2) levels and fertilization rates on the success of IVF/ICSI in infertile women.

Materials and Methods: The study comprised one hundred and twenty infertile women undergoing IVF/ICSI treatment.  Hormone levels were measured using enzyme-linked immunosorbent assays (ELISA). The fertilization rate (FR) was estimated by dividing the fertilized oocytes by the total number of microinjection oocytes. Based on the IVF/ICSI outcomes, participants were categorized into pregnant and non-pregnant groups.

Results: The pregnant group had a significantly higher mean E2 level (1480.84 ± 564.29) than the non-pregnant group (1147.84 ± 440.56) (p < 0.001). There were significant correlations between baseline E2 levels and IVF/ICSI outcomes (r = -0.317, p < 0.001). Furthermore, the pregnant group had a significantly higher mean of FR (72.22 ± 9.56) than the non-pregnant group (44.88 ± 37.17) (p < 0.001). Additionally, significant correlations were found between FR and IVF/ICSI outcomes (r = -0.420, p < 0.001).

Conclusion: Higher E2 levels and higher FR significantly affect the probability of success in IVF/ICSI treatments. More research is needed to substantiate these findings and clarify the underlying processes at work.

A Prospective study Correlation between Serum Estradiol levels and Fertilization Rates in Assisted Reproductive Technology Outcomes

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