Vol.8 No.4 – 6:Potential Role of Circulating Dermokine and Bcl-2 Anti-apoptotic Protein in Colorectal Cancer Egyptian Patients: Correlative Analysis with the Clinicopathological Parameters

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By: Shaimaa M. Abdelsamea1, Hoda M. El-Emshaty2, *, Othman A. Othman1, Mohamed M. El-Hemaly2, Hisham Ismail1

1Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia 61519, Egypt.

2Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.

Running title: Circulating Dermokine and Bcl-2 in CRC Egyptian Patients

Abstract:

Colorectal cancer (CRC) is the seventh most common cancer in Egypt, and more than half of the patients are under the age of 50. Here, we aimed to assess the levels of circulating Dermokine (DMKN) and cytoplasmic anti-apoptotic protein, Bcl-2 for detecting CRC in the earlier stages possible.The levels of DMKN, Bcl-2, Carcinoembryonic antigen (CEA), and Carbohydrate antigen 19.9 were determined using ELISA in the sera of 53 CRC patients, 18 ulcerative colitis patients, and 24 healthy individuals. Statistical analyses were performed using the SPSS program. Serum levels of DMKN and Bcl-2 were significantly higher (p < 0.0001) in CRC patients than in non-cancer individuals. Highly significant correlations were recorded between levels of DMKN and Bcl-2 and the pathological TNM tumor characteristics. At the best cut-off level (68-pg/mL), the DMKN assay showed high degrees of sensitivity (87%), specificity (100%), and accuracy (91%) in comparison with investigated biomarkers. Furthermore, regression analysis revealed a DKB-Score based on DMKN and Bcl-2 with an AUROC of 0.991. The developed score showed a high degree of efficiency (97.4%) for discriminating CRC patients from controls. In conclusion, the assessment of serum DMKN either alone or simultaneously with Bcl-2 has a potential role in discriminating CRC from premalignant patients.

Potential-Role-of-Circulating-Dermokine-and-Bcl-2-Anti-apoptotic-Protein-in-Colorectal-Cancer-Egyptian-Patients-Correlative-Analysis-with-the-Clinicopathological-Parameters-2

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Vol.8 No.4 – 5:Ameliorating effect of extracted Ginger oil against toxic effects of crude oil in male rats

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By: Anas, S. Abuali

Biology Department / Basic Education College / Missan University, Iraq

Abstract

Aim of the study: Use extraction of medical herbal such as powder ginger extract observed to stimulate the hematological system as evidenced by a decrease in the total count of RBCs, platelets as well as hemoglobin percentage. This study aimed to determine the antioxidant activity and protective effect of ginger essential oil against the toxic effect of crude oil on the hematological parameters.

Method: Crude oil was taken from the medical laboratory center in Missan province. Crude oil obtained from Missan oil company (MOC) Ltd sample type (No:38DI) Ginger (Zanjabar offcienales) was obtained from the local market, Missan city, and classified in the Department of Biology, Faculty of Science.

Experimental animals: Twenty-four Albino Waster male rats weighing (150-200 g) were assigned into three groups (8 rats for each group). Group one was administered corn oil at a dose of 1ml for each rat daily basis for 30 days, group two was treated with ginger essential oil orally (50 mg/kg B.W.) once daily for 30 days, group three was treated orally with 600ul of crude oil mixed with 1 ml corn oil orally once daily for 30 days. Blood samples were collected in clean class tubes with EDTA anticoagulant. Complete blood pictures (CBC) shown from collected blood samples by automatic method (Celltac X kx 021n automated hematology analyzer, Japan CARE Co, LTD). The results: The results observed that ginger essential oil is composed of 55 chemical components identified according to retention times and area parentage area in the extracted oil. The main active chemical components were Zingiberen in percentage (17.1), followed by Cyclohexene (α-Sesquiphellandrene) in percentage (12.1%), à-Farnesene in percentage (11.9%) and Benzene (7.9%). Red blood cells (RBCs) observed a significant decrease in the count of red blood cells (RBCs) when administrated crude oil orally as a compared control group, while the rats that were given ginger essential oil observed no significant changes in RBC count. Decrease in hemoglobin concentration (Hb), Packed cell volume (PCV), and Platelets(Plt)after administrated of crud oil in dose 600ul, while the rats that administrated GEO observed no significant changes in Hb, PCVand Plt as compared with the control group. A significant increase in White Blood Cells(WBCs), Granulocytes (Neutrophils), Monocytes, and Lymphocytes after administration of crude oil as compared with the control group, while administration of GEO orally improved activity of WBCs, Granulocytes, Monocytes, and Lymphocytes.

Conclusions: Exposure to crude oil may lead to abnormal changes in the hematological parameters and Ginger essential oil may have properties for protecting and ameliorating the toxic effects of crude oil even in high doses.

Ameliorating-effect-of-extracted-Ginger-oil-against-toxic-effects-of-crude-oil-in-male-rats

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Vol.8 No.4 – 4:Evaluation of ameliorating role of avocado Persea americana fruit extract against monosodium glutamate-induced toxicity in pregnant female albino rats and their offspring

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By: Eman H Radwan1,2*, Abdelfattah Elbeltagy1, R Ibrahim1, Gh Tabl3 and Noha Nazeh1

1Zoology Department, Faculty of Science, Damanhour University, Egypt

2Member of National Biotechnology Network (ASRT), Egypt

3Zoology Department, Faculty of Science, Tanta University, Egypt

Abstract

Background: Although monosodium glutamate (MSG) is commonly used as a food additive, the application of higher doses or prolonged uses significantly leads to accumulations in living cells and finally produces cellular toxicity. Persea Americana (avocado) has recently gained substantial popularity and is often marketed as a “superfood” because of its unique nutritional composition, antioxidant content, and biochemical profile. Aim: To evaluate the potential ameliorative role of avocado fruit extract against MSG-induced nephrotoxicity in pregnant rats and their offspring. Thirty-two (24 females and 8 males) albino rats were used in this study. After an acclimatization period of two weeks; the animals were mated, and the pregnant rats were randomly divided into four groups; control (G1), avocado (G2): they were supplemented with 50 mg/kg b.w. of avocado fruit extract, MSG (G3): they were given 3g / kg b.w. of MSG, every other day, and MSG &Avocado (G4): they were given an oral dose of MSG alternatively with avocado fruit extract. At the end of weaning, the female rats and their offspring were sacrificed and the blood was collected and the kidneys were excised to evaluate the renal biochemical and histopathological, and immunohistochemical investigations. Results: In MSG-treated mothers’ rats, the renal cortical sections displayed severe histopathological lesions including little renal corpuscles, atrophied glomeruli, and relatively wide Bowman‘s space. However, the offspring displayed mild renal histopathological lesions compared with their mothers. The immunohistochemical results revealed strong PCNA and Bax expression in the renal tissues of MSG-exposed mother rats and their offspring if compared with the control.  Furthermore, the mean percentage value of positively expressed cells for caspase-3 appeared significantly higher in the renal cells of MSG-induced mother’s rats and their offspring if compared with the control. Additionally, the levels of serum antioxidants (SOD&CAT) and potassium ions appeared significantly lowered while the level of MDA, urea, and creatinine appeared significantly higher if compared with the control.  Co-supplementation of avocado fruit extract to MSG-induced mothers rats and their pups successfully alleviated the histopathological, immune-histo-chemical, apoptotic as well as biochemical changes caused by MSG.  Conclusion: Avocado fruit extract has a powerful ameliorative role against MSG-induced renal toxicity in mother rats and their offspring.

Evaluation-of-ameliorating-role-of-avocado-Persea-americana-fruit-extract-against-monosodium-glutamate-induced-toxicity-in-pregnant-female-albeno-rats-and-their-offspring

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Vol.8 No.4 – 3:Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies

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By: Rania Shams El Deen Fakher El Deen1, Sabry Ali El-Naggar2, Eman El-Nahass2,Karim Samy El-Said3

1 Nutrition and Food Science, Faculty of Specific Education, Tanta University, Egypt

2 Zoology Department, Faculty of Science, Tanta University, Egypt

3 Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Egypt

Abstract

Background: Natural products demonstrated potential for use in medicine and pharmaceuticals. Objective: This study evaluated the phytochemical composition of Urtica sp. leaves extract (USLE), and its in vitro and in vivo antitumor effects. Methods: HepG-2 and MCF-7 cell lines were used to evaluate the IC50, apoptosis, and cell cycle analysis of USLE. Forty female CD-1 mice were equally divided as follows; Gp1 was control; Gp2, Gp3, and Gp4 were inoculated with 1 × 106 of Ehrlich ascitic carcinoma (EAC)-cells/mouse. Then, Gp3 had injected with Cisplatin (2 mg/kg) intraperitoneally (i.p). Gp4 had injected with USLE (100 mg/kg) i.p. as in Gp3. Total tumor volume, total tumor cell count, and live and dead EAC-cells were determined, also biochemical and histopathological investigations were evaluated. Results: Data showed that the USLE had cytotoxic and antitumor effects against the HepG-2, MCF-7, and EAC-bearing mice. Biochemical and histopathological investigations showed an improvement in the liver and kidney tissues upon treatment of EAC-bearing mice with USLE. Conclusion: The results showed that the USLE can stop tumor growth and cause tumor cells to die.

Antitumor-efficacy-of-Urtica-sp.-leaves-extract-in-vitro-and-in-vivo-studies

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