Vol.8 No.4 – 2:Non-invasive follow-up of Egyptian patients infected with Helicobacter pylori by quantification of H. pylori circulating antigen in serum using ELISA

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By: Hager R. Fawzy1, Asmaa M. Abdelmageed2, Mahmoud A. Shoulkamy1, Mohamed Abdel Wahab2, Hisham Ismail3, *

1Microbiology Division, Botany & Microbiology Dept., Faculty of Science, Minia University, Minia 61519, Egypt

2Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt

3Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia 61519, Egypt.

Abstract

Clinicians still wish to determine if H. pylori-infected patients have been cured after specific treatment. The present study aimed to evaluate the reliability of the H. pylori circulating antigen (HpCAg) test for noninvasive screening of H. pylori infection and assessment of cure after specific treatment. Sera of 134 symptomatic individuals (81 males & 53 females, aged 23-68 yr) were screened for HpCAg using ELISA. H. pylori infection was confirmed using a gold standard based on culture, rapid urease test, and histology testing. The detection rate of HpCAg was 69% among screened individuals. The gold standard confirmed H. pylori infection in 93% of individuals showing HpCAg in their sera. In addition, 31% of infected patients were excluded for their drug resistance. Eligible individuals received a standard triple therapy regimen including Lansoprazole, Clarithromycin, and Amoxicillin twice daily for 14 days. Six weeks later, the HpCAg testing was repeated to evaluate the treatment outcome. HpCAg was not detected in 78 % of treated individuals. Furthermore, the levels of HpCAg were significantly decreased (p < 0.001) in the sera of non-responders. In conclusion, the detection of HpCAg is a reliable non-invasive approach for screening and follow-up of H. pylori-infected individuals after treatment, especially in developing countries.

Non-invasive-follow-up-of-Egyptian-patients-infected-with-Helicobacter-pylori-by-quantification-of-H.-pylori-circulating-antigen-in-serum-using-ELISA

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Vol.8 No.4 – 1:The hepato-fibrogenic potential of both acute and chronic treatments with paracetamol, ibuprofen, and aspirin in rats

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By: Nabil Mohie Abdel-Hamid l, Mona Mohammed Abdel Hamid 2, Ahmed Abdelnaser Mohamed*3

1Department of Biochemistry, Faculty of Pharmacy, Kafrelsheikh University, Egypt,

2Department of Biochemistry, Faculty of Pharmacy (for Girls), Al-Azhar University, Egypt, Orcid ID: 0000-0002-2810-7016.

3Department of Biochemistry, Faculty of Pharmacy, New Mansoura University, Egypt, Orcid ID: 0000-0003-0666-234X.

ABSTRACT

Background and purpose: Hepatotoxicity from frequently prescribed drugs has become an evolving health problem. This study was conducted to evaluate the risk of acute and chronic administration of acetaminophen (AAP), ibuprofen (Ibu), and acetylsalicylic acid (ASA). Methods: One hundred and twenty male albino rats, were divided into 2 main groups for acute and chronic study. Each group was sub-classified into 5 sub-groups (12 rats for each). Acute study: control (normal saline), AAP (single oral dose, 540 mg/kg, bw), AAP +Zn (APP and Zn ,227 mg/liter drinking water 24 hours before AAP administration), Ibu (single oral dose,440 mg/kg, bw), and ASA (single intraperitoneal dose,540 mg/kg, bw). Chronic (period for 60 days): control (normal saline), AAP (single daily doses, 48 mg/kg, bw), AAP +Zn (APP and Zn, 227 mg/liter drinking water for 6o days), Ibu (single daily doses, 48 mg/kg, bw), and ASA (single daily intraperitoneal doses, 40 mg/kg,). Results: Hepatic aminotransferases, alkaline phosphatase, isocitrate dehydrogenase, serum glycosaminoglycans, tissue hydroxyproline, and malondialdehyde were significantly elevated, but glutathione was significantly decreased, in both acute and chronic treatments in all treated groups. Prior treatment with Zn couldn’t change the effects of AAP, except on oxidative stress. Tissue changes after chronic treatment varied from fatty changes to vascular congestions and inflammation. Conclusion: We assume that both acute and chronic administration of AAP, Ibu, and ASA have deleterious hepatotoxic and fibrogenic effects on the liver with a non-significant protective role to Zn co-administration with AAP against oxidative stress.

The-hepato-fibrogenic-potential-of-both-acute-and-chronic-treatments-with-paracetamol-ibuprofen-and-aspirin-in-rats

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