Vol.8 No.3 – 7:Detection of DNA damage by SCD and Rate of Apoptosis DNA by Gel Electrophoresis among infertile males

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By: Nehad Nabil Eskarous1, Sobhy El-Sayed Hassab El-Nabi1, Mohamed Ahmed Abd El Salam2, Khaled Geba1, and Sameh Fayek GamalEl Din2

1- Genetic Engineering and Molecular Biology Department of Zoology, Faculty of Science Menoufia University, Egypt

2- Department of Andrology, Sexology & STDs, Faculty of Medicine, Cairo University, Egypt

Abstract:

Background: DNA damage as Fragmentation has adverse effects on fertilization and embryo development, so it is one of the main causes of a male factor for infertility. Several techniques have been mentioned to elevation this damage. In our study, we determine DNA damage in human spermatozoa by sperm chromatin dispersion (SCD) method and Apoptosis of DNA in human spermatozoa by Optical density in gel electrophoresis in male infertility. Objects and Methods: Semen samples were collected from 100 men and were analyzed by standard light microscopic according to the World Organization (5th edition) for diagnostic fertility. Furthermore, Sperm DNA damage was determined by using Halosperm Kit, then assessment apoptosis by optical density in Gel Electrophoresis. Results: The mean value of DNA by SCD method in infertile males increased with a value of 47.95±10.96 %   when compared with the control value of 21.2 ±2.64 % with (p< 0.00001). On the other hand, the mean value of DNA by measurement of Optical density in Gel Electrophoresis in infertile males decreased with a value of 120.27±18.73 when compare with the control value of 144.4±45 with (p =0.833). Conclusion: The assessment of sperm DNA damage by SCD method and other methods for detection of DNA apoptosis by gel electrophoresis addition to routine semen analysis play important role in the diagnosis and management of male infertility. 

Detection-of-DNA-damage-by-SCD-and-Rate-of-Apoptosis-DNA-by-Gel-Electrophoresis-among-infertile-males

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Vol.8 No.3 – 6:Antimicrobial activity of Lactobacillus acidophilus against pathogenic Escherichia coli isolated from diarrhea patients

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By: Furqan Majid Kadhum

Department of Medical Optics Techniques, Al-Farabi University College, Baghdad, IRAQ

Abstract:

Lactobacillus acidophilus represents a probiotic bacterium that may be found in the mouth, gut, and vaginal canal. Lactobacillus acidophillus may help to relieve diarrhea, bloating, and cramps caused by bacteria that may cause everything from diarrhea to life-threatening colon inflammation. Escherichia coli (hencefourth E. Coil) bacteria are found in healthy people’s intestines as natural flora. Only a very small percentage, like the E. coli O157:H7 variety, can lead to severe stomach discomfort, bloody diarrhea, and vomiting; the rest of the E. coli variety is harmless.

Antimicrobial activity of Lactobacillus acidophilus against pathogenic Escherichia coli isolated from diarrhea patients (1)

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Vol.8 No.3 – 5: Stem cell therapy in renal diseases

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By: Khalil A. Alhalfawy 1, Bahgat A. Elfiky 2, Ahmed M. Zahran 3, Zeinab A. Kasemy 4 and Mahmoud M. Zayed 5

  1. Molecular biology – Genetic Engineering and Biotechnology Research Institute, University of Sadat City
  2. Animal Biotechnology – Genetic Engineering and Biotechnology Research Institute, University of Sadat City
  3. Internal medicine & Nephrology Faculty of medicine – Menoufia University, Egypt
  4. Public health and community medicine – Faculty of Medicine – Menoufia University, Egypt
  5. Researcher – Animal Biotechnology – Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt

Abstract

Objective: to evaluate the stem cell in treating renal diseases. To evaluate the effect of stem cells on renal cell apoptosis and necroptosis. Background: The use of stem cells is the hope for all patients with end-stage renal diseases. We isolate stem cells from umbilical cord blood to treat renal diseases by using the Vero cell line as a renal cell which is treated by hydrogen peroxide as renal failure. P53, RIPK1, and EGFR genes were detected by RT PCR to show the improvement by using stem cell therapy for renal diseases. Mesenchymal stem cells are the future hope for the treatment of renal diseases associated with renal cell apoptosis and necroptosis.

Methodology: Mesenchymal CD105 Stem Cells Separation from Umbilical Cord Blood from 20 pregnant females. By forming buffy coat mononuclear leukocytes. Magnetic labeling of CD105 and Separation to stem cells. stem cell proliferation by using Dulbecco Modified Eagle medium (DMEM). After proliferation passages 1, 2, and 3 freeze cells at -20c. For one week then thawing at room temperature to make stem cell extraction. Vero cell line treated by H2O2 1.6 mm for 5 hours to reach sub-lethal. The cytotoxicity of the cell was caused by hydrogen peroxide measured by MTT assay and Spectrophotometry. The Vero cell line is divided into 4 groups group1: normal control, group 2:  sub-lethal, group 3: sub-lethal treated by stem cell extraction, and group 4: sub-lethal treated by stem cells. P53, RIPK1, and EGFR genes were detected in 4 groups by RT PCR. Results: P53, RIPK1, and EGFR levels showed a highly significant difference among studied groups with elevated levels of P53 and RIPK1 and reduced levels of EGFR in the sub-lethal renal cell group with an improvement of cells treated with mesenchymal stem cells. Mesenchymal stem cells showed better results when compared with mesenchymal stem cell extract. Conclusion: Mesenchymal stem cells demonstrated a good effect on renal cell line injury, apoptosis, and necroptosis.

Stem-cell-therapy-in-renal-diseases

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Vol.8 No.3 – 4: Genetic study of I kappa B alpha gene promoter polymorphism associated with hepatitis C virus in Egyptian patients

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By: Mamoun Kamal Ghazalah 1, Sameer El-Masry 1, Ibrahim Helmy2, and Ehab Abd-Elkhalek3

  1. Department of Molecular Biology, Biogenetic engineering Research Institute, Sadat City University, Egypt
  2. Department of Molecular Biology Faculty of Science, Kafr al-Sheikh University, Egypt
  3. Department of Gastroenterology and Endoscopy, Faculty of Medicine, Mansoura University, Egypt

Abstract

Background: Host genetic polymorphism is one of the major unalterable major factors for HCV infection, NF-κB proteins play multiple roles in immune response and involve in HCV infection and progression.

Aim of the study: To investigate the associations between single nucleotide polymorphism (SNPs) in NF-Kb and the susceptibility as well as resolution of HCV infection. Patients and Methods:  This prospective case-control study was conducted at the physical examination center on 150 Egyptian population, including 50 uninfected control cases, 50 cases with spontaneous viral clearance, and 50 cases with persistent HCV infection, they are genotyped for four SNPs (rs11820062, rs230530, rs1056890 and rs3774963) using a Taq Man assay.  Results: The current study revealed that the mutation in rs_11820062 of the I kappa B alpha gene significantly increased the risk for HCV infection with a p-value <0.05. Conclusion: This study revealed that genetic variants of the NF-κB pathway genes (rs11820062 T allele) are associated with an increased risk of HCV susceptibility.

Genetic study of I kappa B alpha gene promotor polymorphism associated with hepatitis C virus in Egyptian patients (2)

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