Vol.8 No.2 – 3: Histopathological study of thyroid carcinoma in Misan province

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By: Osamah Nassir Wali1, Abbas Ch. Mraisel2, and Alaa N. Salih3          

1- Histology / Basic Medical Science Department / Nursing College / Misan University, Iraq

2- Toxopathology / Basic Medical Science Department / Nursing College / Misan University, Iraq

3- Histology /Biology Department /College of Education for Pure Sciences /Wasit University, Iraq

Abstract

Aims: “Thyroid carcinoma is specific malignant tumors derived from follicle cells including papillary and follicular carcinoma, and parafollicular, calcitonin-producing C-cells (medullary carcinoma)”. This study aimed to provide a present outlook on thyroid carcinoma among patients and investigation the relationship between the age, sex, and thyroid carcinoma in Missan province in Iraq. Methods: The data were collected in the period (First June – 2020 to Thirty November -2020) from (42) patients aged between (35 -80 or more) infected with thyroid carcinoma, a histopathological examination that performed and confirmed in the tissue sample obtained from the patients after surgical operations in AL-Sadder hospital in Missan province. Results: “The patients with thyroid carcinoma observed high incidence in ages between (41-55) years (42.8 %) cases, followed by the ages between (56-70) years (28.5 %) cases, while the lowest incidence observed in ages between (71or more) years (11.9%)cases. Thyroid carcinoma is observed more commonly found in females (64.28%) cases than the males (35.71%) cases (P < 0.05). Histopathological examination of the tissue specimens shows the commonest type of thyroid carcinoma was the papillary carcinoma” 28(66.6) cases characterized by multifocal with papillary architecture, changes in the size of thyrocytes with an abnormal nucleus and cytoplasm and shape, followed by follicular carcinomas 9(21.4) cases characterized by presence malignant epithelial cells arising from follicular cells and invasion into surrounding thyroid tissue, while the lowest type was Medullary carcinoma  5(11.9)cases, showing islands of tumor cells with central necrosis surrounded by vascular strom. Conclusion: “Papillary carcinoma is the most common malignant tumor of the thyroid gland and as compared with follicular carcinomas and medullary carcinoma. Proper implementation of preventive measures such as changing lifestyle factors might enhance control of thyroid carcinoma”.

Histopathological study of thyroid carcinoma in Misan province-converted (2)

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Vol.8 No.2 – 2: Frequency and specificity of red blood cells alloantibodies among Sudanese multiparous women

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By: Nour Mahmoud Abdelateif Ali 1, and Abather Alsayed Mohamed 2

  1. Department of Hematology, Faculty of Medical Laboratory Sciences, Alneelain University, Khartoum, Sudan
  2. Department of Hematology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Khartoum, Sudan

Abstract

Background: Allo-immunization is a condition in which the body gains immunity, from another individual of the same species, against its cells. Blood group antibodies are called alloantibodies if they react with alloantigens on the red blood cells (RBCs) of other individuals. Immune antibodies are produced following exposure to foreign erythrocyte antigens through pregnancy or transfusion. Maternal alloimmunization, also known as iso-immunization, occurs when a woman’s immune system is sensitized to foreign erythrocyte surface antigens, stimulating the production of immunoglobulin G (IgG) antibodies. Purpose: This study aimed to detect the frequency and specificity of alloantibodies against (RBCs) antigens among Sudanese multiparous women.

Methods: This cross-sectional study was conducted on randomly selected 130 pregnant ladies, most of them were attending Soba teaching hospital and the remaining to Khartoum teaching hospital during the period from February-2008 to May-2008. Serum from each lady was collected. Each sample was grouped and screened for alloantibodies, then samples with positive antibody screening were tested for antibody identification using the gel agglutination method. Results: The overall frequency of alloantibodies detected among studied pregnant ladies was 10.8%. The results also revealed that an insignificant correlation was found between age, ethnic group, and presence of alloantibodies ((P-value: > 0.05); While a significant correlation was observed between the history of abortion, and the number of pregnancies and presence of alloantibodies (P-value: < 0.05).

Conclusion: In summary, we concluded that the alloantibodies were detected in one-tenth of the studied subjects with a significant association between detected antibodies and the number of pregnancies.

Frequency and specificity of red blood cells allo-antibodies among Sudanese multiparous women-converted

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Vol.8 No.2 – 1:Induction of renal cell line apoptosis by hydrogen peroxide

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By: Khalil A. Alhalfawy1, Bahgat A. Elfiky2, Ahmed M. Zahran3 and Mahmoud M. Zayed2

  1.  Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt
  2. Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute – University of Sadat City, Egypt
  3. Internal medicine and nephrology Department, Faculty of medicine – Menoufia University, Egypt

Abstract

Background: Hydrogen peroxide is currently the most widely used as an apoptosis inducer due to its broad cytotoxic efficacy against nearly all cell types. cytotoxicity is achieved over a wide range of doses

Objective: To evaluate the cytotoxic effect of hydrogen peroxide on renal cell lines by detecting RIPK1.

 Methods: In this study, we used a Vero cell line treated with H2O2 at concentrations 0.1 mM, 0.2 mM, 0.4 mM, 0.8 mM, and 1.6 mM and examined after 30 min,1hour,2hours,3hours, 4hours and 5 hours. by using MTT assay to detect cytotoxicity to cell line (by detecting cell viability). Spectrophotometrically measure the absorbance at a wavelength of 570 nm. Measure the background absorbance of multi-well plates at 690 nm and subtract from the 450 nm measurement. Sub-lethal dose to renal cell line is one treated with 1.6 mM for 5 hours. groups group 1: renal cell line as control which not treated by H2O2. group 2: Sub-lethal which renal cell line treated by concentration 1.6 mM of H2O2 for 5 hours. Results: H2O2 is cytotoxic to renal cell line by concentration from 0.1 mM to 1.6 mM. RIPK1 gene expressed in renal cell line treated by H2O2. The sublethal dose reached 1.6 mM for 5 hours.  There is a significant difference between the 2 groups by detecting the expression of the RIPK1 gene.

Induction

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