Vol.6 No.2 – 5: Role of Human Pituitary Tumor-Transforming Gene1 (HPTTG1) as a prognostic biomarker for metastasis in Egyptian patients breast, colon and Liver cancer

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By: Samir Ali Mohamed El-Masry1, Sherif Mohsen Elsherbini1,

Abdelmoneam Ahmed Abdelmoneam2, Ahmed Gouda Ibrahim Radwan1

1-Molecular Biology department, Genetic Engineering, and Biotechnology Research Institute, Sadat City University, Egypt, 32897

2- Haemato-Oncology department, Faculty of Medicine, Banha University, Egypt, 13511

Abstract

hPTTG1 is an oncogene that overexpressed in most human carcinomas. It was reported to be involved in cell cycle regulation and sister chromatid separation.  PTTG expression level has been associated with tumor progression, invasion, and metastasis. In this study, hPTTG1 expression was analyzed in three cancer patients’ groups including; breast cancer, colorectal cancer, and hepatocellular carcinoma patients using RT-PCR. Our data revealed that the expression of PTTG1 was high in three groups; however, the metastatic cases reported higher expression levels of PTTG1 compared to non-metastatic groups. Thus, PTTG1 could be a prognostic marker in different types of cancer patients, and targeting PTTG1 might be a good strategy against metastasis.

Role of Human Pituitary Tumor-Transforming Gene1 (hPTTG1) as a prognostic biomarker in Egyptian patients breast, colon and liver cancer.

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Vol.6 No.2 – 4: Effect of Polyethylene and Butylated Hydroxytoluene on the histological structure and some enzymes of rat liver

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By: Hassan Al-Harbia, Zaki Al-Hasawib, Yaser Binnaserc, Reem Al-Hasawid , Faiz Al-Solamia  and Abdullah Al- Ghamdia

aPoison Control and Medical Forensic Toxicology Center, Jeddah, Saudi Arabia

bDepartment of Biological Sciences, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia

cDepartment of Biology, College of Sciences, Taibah University, Al-Medina Al-Munawara, Saudia Arabia

dDepartment of Chemistry Sciences, Faculty of Science and Arts, King Abdul Aziz University, Rabig, Saudi Arabia

Abstract

This research studied the effect of polyethylene (PE), Butylated Hydroxytoluene (BHT), PE with BHT, and the thermally-treated PE on the histopathological examination and some biochemical activities of the liver of adult male mice.105 mice were divided into 5 groups. The (PE group) supplied with 20% crushed PE, the (BHT group) supplied 400mg/kg of BHT dissolved in corn oil, the (PE + BHT group) were fed with 20% crushed PE and 400mg/kg of BHT dissolved in corn oil, the (thermally treated PE group) supplied food with 20% of crushed thermally-treated PE, and the control group.  The weight of the mice was recorded before the study and after 6 weeks. Blood samples were collected to determine the liver enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The animals were sacrificed; the liver was weighed before and after treatments and examined microscopically. Liver specimens were stained and examined by a light microscope. The results showed that the mean weights of the mice in PE, BHT, and PE + BHT groups were statistically reduced and the mean weights of their livers significantly (p≥0.05) increased and the enzymes AST and ALT in the blood significantly (p≥0.05) increased in comparison to the control group. The histopathological examination of the liver specimens showed significant changes with all PE and BHT treatments. The results of the current investigation suggest preventing the use of plastic in food preservation and replacing it with safe grade substance like wood materials

Effect of Polyethylene and Butylated Hydroxytoluene on the histological structure and some enzymes of rat liver-converted

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