Vol.5 No.4 – 5: CD47 Expression in Egyptian Patients with Acute Myeloid Leukemia

By: ¹Samar Saber, ¹Samir A. El-Masry, ²Salah Aref

¹ Molecular Biology Department, Genetic Engineering and Biotechnology Institute, Sadat University, Egypt

² Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt

Abstract

Background: Acute myeloid leukemia (AML) is a genetically heterogeneous clonal disease defined by the proliferation and accumulation in the bone marrow and blood of immature hematopoietic cells. CD47 -Signal Regulatory Protein α (Sirpα) Regulate the negative regulation of phagocytosis, an inhibitory receptor. CD47 overexpression in AML is overexpressed on peripheral blasts and stem cells with leukemia and correlates inversely with survival. Aim: Estimation of CD47 gene expression in Egyptian AML patients for evaluation of its role in the pathogenesis of the disease and as a prognostic marker related to overall survival (OS). Methods: The 55 patients with AML in addition to 21 healthy individuals as a control group were included in the current study. Blood samples from the control group and patients were subjected to a positive magnetic selection of CD34+ cells of leukemic, and then using flowcytometry the CD47 expression on these cells was estimated. Results: Statistically significant difference (P < 0.0001) between two groups AML patients and control group in Hemoglobin (g/dL), WBCs (10³/µL) and PLTs count (10³/µL). The results on incidences of CD47 differ significantly among FAP type in AML patients. The mean percent of CD47 were increased with FAP type excretion, 38.6 ± 39.5 at M1, 51.1±34.9 at M2, 60.2±51.9 at M3 while, those showed decrease 33.1±22.6 with M4 and 43.2±31.4 with M5. There was an inverse correlation between concentrations of CD47 gene expression and general survival, with enhanced expression also connected with worse general survival (P < 0.0001). Conclusion: The results obtained by this study provide additional evidence of the role of CD47 gene as a predictive factor impact on prognosis and OS.

CD47 Expression in Egyptian Patients with Acute Myeloid Leukemia-converted

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