Vol.5 No.2 – 10 : Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats

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By: Asmaa F. Galal1*, Lamiaa M. Salem2, Mahrousa M. Hassanane2, Somaia A. Nada3,  Omar M. E. Abdel-Salam1 

1 Narcotics, Ergogrnics and Poisons Department, National Research Centre, Giza, Egypt. Affiliations ID: 60014618.

2Department of Cell Biology, National Research Centre, Giza, Egypt. Affiliations ID: 60014618.

3 Pharmacology Department, National Research Centre, Giza, Egypt. Affiliations ID: 60014618.

Abstract

Objective: In the present study, we explored the therapeutic potential of citicoline in preventing malathion induced neurotoxicity and genotoxicity. Citicoline has been shown to act as a neuroprotective in experimental animal models of ischemia and other types of brain injuries.Methods: Acute malathion intoxication was induced by intraperitoneal injection of malathion (150 mg/Kg, once per day) for two successive days. Citicoline was co-administered in three doses (100, 200, 300 mg/kg, p.o.). Serum Butyrylcholine esterase (BChE) and Paroxonase-1 (PON-1) were assessed as exposure biomarkers. Oxidative stress biomarkers were assessed in different brain region (cortex, striatum, and subcortex) in addition to TNF-α. Genotoxicity was tested by chromosomal aberrations, mitotic index, DNA fragmentation, and micronucleus tests. Results: Malathion administration resulted in marked suppression of serum BChE and PON-1 activities. Also, the exposure  to the pesticide led to elevated malondialdehyde (MDA), nitric oxide (NO) and decreased reduced glutathione (GSH) levels in investigated brain regions in addition to elevated striatal tumor necrosis factor-α (TNF-α) level. Malathion also caused profound structural chromosomal aberrations, increased liver DNA fragmentation and mitotic index. These effects were alleviated with administration of citicoline dose dependently. Conclusion: Our data indicate that citicoline can protect against malathion neurotoxic and genotoxic potential, possibly through antioxidant, anti-inflammatory activities and restoring energy stores.

Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats-converted

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