Vol.5 No.2 – 7 : Combining nuclear matrix protein-52, collagen III and matrix metalloproteinase-1 for more effective breast cancer early detection

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By: Abdelfattah M. Attallah,1* Mohamed El-Far,2 Mohy E. Abdel Fatah,3 Mohamed M. Omran4, Mohamed A. Abdelrazek,1  Gamal E. Abdelhameed,1 Kareem A. Attallah,1 Nada A. Ahmed,1 Esraa A. El-sayes,1 Fatma M. Khedr,1 Ibrahim El-Dosoky 5

1Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

2 Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt

3 Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

4 Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt

5 Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Abstract

Aim: Improvement breast cancer (BrCa) control will be markedly supported by early detection. Owing to limitations of current diagnostic tools like mammography and ultrasound and lack of existing confirmed BrCa biomarkers, this study concerned the evaluation of some potential biomarkers and their combination in BrCa detection. Methods: Three hundred participant women; 200 with BrCa patients, 50 with benign breast diseases and 50 healthy individuals were enrolled in this study. Serum levels of nuclear matrix protein-52 (NMP-52), collagen III and matrix metalloproteinase-1 (MMP-1) were determined by ELISA. Results: Mean levels of NMP-52 (9.83±1.1 μg/ml), collagen III (22.6±3.2 μg/mL) and MMP-1 (3.6±0.3 μg/mL) in BrCa patients were significantly higher (P<0.0001) than benign (5.8±0.7, 12.2±1.3 and 2.6±0.23 μg/mL, respectively) and healthy (1.2±0.1, 6.0±0.2 and 1.66±0.04 μg/mL, respectively) groups. Also, these levels were associated with the tumor progression and may reflect the BrCa disease severity, high serum levels of these markers have been associated with tumor advanced stages (T3-T4), high grade (G3), and large size (>2cm). Diagnostic score combined these markers revealed valuable power (AUC=0.83, 78% sensitivity, 75% specificity) in BrCa diagnosis. This power not markedly influenced in detection of early tumor stages (Tis-T2), low grade (G1-G2), lesser tumor size ≤2 cm and negative lymph nodes status (AUC=0.79, 0.74, 0.74, and 0.85, respectively).Conclusions: Combined use of NMP-52, collagen III and MMP-1 can serve as potential biomarker for BrCa diagnosis. This combination is likely to improve the clinical early tumor diagnosis.

Combining nuclear matrix protein-52, collagen III and matrix metalloproteinase-1 for more effective breast cancer early detection-converted

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Vol.5 No.2 – 6 : Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia and their HBV Infection Status

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By: Abdirauf Mohamed Abdi and Mohd Nazil Salleh

Department of Health Sciences, Faculty of Engineering and Life Sciences, Universiti Selangor, Shah Alam City Campus, Jalan Zikron A 7/A, Seksyen 7, 40000 Shah Alam Selangor, Malaysia

Abstract

Background: Hepatitis B virus (HBV) infection is a serious health problem that can lead to liver cirrhosis and cancer. Assessing the public knowledge, attitude and practice towards HBV infection can be useful in planning public health policies in HBV management. Objective: The current study sought to determine the association between knowledge, attitude, and practice towards HBV infection with the HBV infection status of Somalian immigrants in Malaysia. Method: A cross-sectional study was conducted from September 2017 to April 2018 among Somalian immigrants in the state of Selangor, Malaysia. Data was collected using validated, self-administered structured questionnaire. Their blood samples was collected for detection of HBV DNA using nested PCR. Descriptive statistics and chi square analysis were done to determine this association. Result: A total of 145 participants were recruited. Majority of participants were in the age group of 20-31 (84.1%), male (62.8%), single (52.4%), had educational level of undergraduate degree (70.3%), and unemployed (88.3%). Majority of the participants show good knowledge (82.8%) and attitude (78.6%) but generally poor practice (32.4%) towards HBV infection. The mean knowledge, attitude and practice score among them were 16.9 ± (4.89), 9.20 ± (2.94) and 9.39 ± (2.93) respectively. The sociodemographic characteristic variables and KAP of HBV were not significantly associated. Selected samples collected from the participant tested negative for HBV DNA via nested PCR assay. Conclusion: Somalian immigrants in Malaysia have a good level of knowledge and attitude but poor level of practice towards HBV infection. All participants were negative for HBV infection.

Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia and their HBV Infection Status

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Vol.5 No.2 – 5 : Molecular expression and single nucleotide polymorphisms of IL17A gene among etanercept-treated rheumatoid arthritis patients

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By: Aseel S. Mahmood1, Abdul-Kareem A. Al-kazaz1, Khadier Z. Mayouf2 and Ali H. Ad’hiah3

1Biotechnology Department, College of Science, University of Baghdad, Baghdad, Iraq.

2College of Medicine, University of Baghdad, Baghdad, Iraq.

3Tropical-Biological Research Unit, College of Science, University of Baghdad, Baghdad, Iraq.

Abstract

Molecular expression (reverse transcription-quantitative polymerase chain reaction; RT-qPCR) and DNA-sequencing-based single nucleotide polymorphisms (SNPs) of interleukin 17A (IL17A) gene were determined in 51 etanercept-treated Iraqi rheumatoid arthritis (RA) patients and 45 control. The results revealed that the relative expression (2-∆∆Ct) of IL17A gene was increased by 1.28 ± 0.29 fold in RA patients, and such profile was approximated in male (1.66 ± 0.58) and female (1.01 ± 0.28) patients.  With respect to PCR-amplified DNA sequences, out of the 10 encountered SNPs, two SNPs (rs8193038 and rs3819025) showed allele frequencies that exceeded 10%. The rs8193038 SNP allele and genotype frequencies showed no significant variations between RA patients and control. The second SNP (rs3819025) was observed to have three genotypes (AA, AG and GG). Among these genotypes, it was observed that the homozygous genotype of mutant allele (GG) was only recorded in patients with a frequency of 13.7%, while none of the control had this genotype. Such difference was significant even after the correction of probability (pc = 0.05), and the associated OR was 15.34 (95% C.I.: 1.39 – 169.24). It was also observed that G allele showed a significant increased frequency in patients (25.5 vs. 12.2%; OR = 2.46; 95% C.I.: 1.14 – 5.30; p = 0.015), while A allele frequency was significantly decreased (74.5 vs. 87.8%; OR = 0.41; 95% C.I.: 0.19 – 0.88; p = 0.015). However, the significance in both cases was lost when the probability was corrected.  It was also observed that there was no significant impact of the rs3819025 SNP genotypes on expression of IL17A gene. In conclusion, IL17A gene showed an increased expression in RA patients, and rs3024419 SNP is suggested to be associated with an increased risk to develop the disease in Iraqi population.

Molecular-expression-and-single-nucleotide-polymorphisms-of-IL17A-gene-among-etan

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