Vol.5 No.1 – 12 : Gelatin-coated magnetic nanoparticles-based DNA isolation method: A comparison with commercial DNA isolation kits from whole blood

By: Kelly S Levano^1,2, Luis Jaramillo-Valverde^1,2, Elizabeth Delgado^1,2, Heinner Guio^1,2*

¹INBIOMEDIC Research and Technological Center, Lima, Peru;

 ²ALBIOTEC, Lima, Peru;

Abstract

DNA isolation is the first step for most molecular tests, thus indicating the importance of using a reliable and suitable method for this procedure. The use of magnetic nanoparticles for blood DNA isolation offers several advantages including: subjecting little mechanical stress to your sample, obtaining higher quality and quantity of DNA and non-laborious procedures. In this study, we isolate genomic DNA using gelatin-coated magnetic nanoparticles from blood samples and test its use compare with three commercial blood DNA isolation kits.  DNA purity and yield were assessed by measuring absorbance at A260/A280 and by agarose gel electrophoresis. The suitability of the isolated DNA for downstream applications was analyzed by end-point PCR and Sanger sequencing. In this study, gelatin-coated magnetite particles for genomic DNA isolation provide an efficient, simple, and inexpensive method that does not require the use of commercial blood DNA Isolation Kit.

Gelatin-coated magnetic nanoparticles-based DNA isolation method A comparison with commercial DNA isolation kits from whole blood

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