Vol.3 No.4 – 11: Nephroprotective Effect of Melatonin against Aluminum Phosphide Induced Renal Tissue Damage in Rats

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By: Mohamed SA. El-Gerbed

Zoology Department, Faculty of Science, Damenhour University, Damenhour, Egypt

Abstract

Aluminum phosphide (AlP) is a cheap, effective and commonly used agricultural pesticide. The present experiment was undertaken to investigate the effect of melatonin against aluminum phosphide -induced renal toxicity in rats. Forty male rats were divided into four groups.  group I: rats maintained as control, group II: rats received melatonin, group III: rats received AlP, group IV: rats received AlP and melatonin with same previous doses. Data showed that AlP “GIII” treatment resulted in a significant increase in serum creatinine and urea level. Also, a markedly significant increase in lipid peroxidation (MDA). On the other hand, the administration of AlP causes a significant decrease in enzymatic antioxidants activities (superoxide dismutase, catalase, glutathione peroxidase glutathione reductase, and glutathione-S-transferase) in the kidney. The histopathological examination of the kidney of AlP -treated group rats, revealed kidney injury with necrotic changes, enlargement of many glomeruli, tubular dilatation and leukocytic infiltration. Electron micrographs of the renal corpuscle showed obvious signs of injury, focal segmental thickening, and podocyte changes, mesangial cells appeared highly deteriorated. Also, proximal convoluted tubules lining cells revealed tremendous alterations and abnormalities in architectural features, an increasing number of the irregular shape of mitochondria with fragmented cristae. Moreover, kidney tissues showed markedly higher p53 induction.  Additionally, treatment with melatonin alleviates the nephrotoxicity of AlP by significantly renormalize the Serum enzymatic and biochemical parameters. The biochemical results were supported by histopathological and ultrastructural observations of the kidney. Moreover, inhibition of p53 with melatonin was seen. From these results, it could suggest that the melatonin might be useful for preventing nephrotoxicity caused by aluminum phosphide through ameliorative effects on (especially parameters) biochemical indices, oxidative stress, histological and ultrastructural changes.

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Vol.3 No.4 – 10 :Morphological and molecular characterization of some olive (Olea europaea) cultivars in El-Arish, Egypt

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BY: Amina Abdel Hamid Mohamed 1*, Mohamed A. Nagaty 2,  Manal M. S. El-Baghdady1  , Khaled H. Radwan 1, 3

1 Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza -12619, Egypt.

2 Plant Production Department, Faculty of Environmental Agricultural Sciences, Arish University, El-Arish, North Sinai, Egypt.

3 National Biotechnology Network for Expertise- Egypt.

Abstract

Morphological characters, along with three different random PCR based markers, (ISSR, SCoT and RAMP) were used to characterize and to assess the genetic diversity among the main nine olive cultivars in El-Arish, Egypt. Analyses of 16 morphological characters revealed the existence of a recorded genetic variability among the studied cultivars. Moreover, 12 ISSR primers, 13 SCoT primers and 11 RAMP primer combinations (PCs) produced 197, 242 and 172 of total loc with 163, 236 and 140 of them being polymorphic respectively. Average polymorphism information content (PIC value) of 0.26, 0.31 and 0.26 detected for ISSR, SCoT and RAMP markers respectively.  Based on morphological and the selected molecular markers, the nine olive cultivars were grouped in two distinct clusters. The highest genetic similarity observed was that between Teffahi and Ageezi, while the least similarity was that recorded between Ageezi Shami and Koroneiki.

The tested marker systems would serve as a complementary tool to provide a more complete understanding of the diversity available in olive populations in Egypt.

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