Vol.3 No.2 – 4 : Investigating the effect of therapeutic ultrasound irradiation on the liver and kidney function of male albino mice

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By : Moustafa Hussein Moustafa

Abstract

In the current explanatory study an experimental methods were conducted to anticipate the effect of therapeutic Ultrasound irradiation on liver and kidney of male albino mice. The histopathological studies revealed a convenient apparent aberration. These finding were supported by hematological investigation of complete blood count (CBC), blood enzymes like aspartate amino transferase (AST or SGOT) and alanine amino transferase (ALT or SGPT), also Creatinine and Urea levels were detected. The results showed no significant alteration in the physiological function of both liver and kidney. The level of malondialdehyde (MDA) and total antioxidant contents were carried out. the level of Glutathione (GST), Superoxide dismutase (SOD) were still in normal ranges.

moustafa 6-2017 vol3 issue2

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Vol.3 No.2 – 3: The evolving schistosomiasis agenda 2017-2020 in Egypt : Moving from control toward final elimination

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By : Ahmed Abdelhalim Yameny

Society of pathological biochemistry and hematology, Egypt

Ahmed A. Yameny (dr.ahmedyameny@yahoo.com)

Abstract

Schistosomiasis is one of the most important diseases of humans in tropical and subtropical parts of the world, it is a  chronic disease, it comes after malaria in socioeconomic and public health importance among parasitic diseases. It is estimated that about 779 million people are at risk of infection, about   240 million are infected, of whom  120 million are symptomatic and 20  million have serious consequences (WHO, 2014). This infection has been recorded in Egypt from early pharaonic times (3200 B.C.) by the demonstration of Schistosome eggs and circulating Schistosome antigens in mummies. After the discovery of the parasite’s life cycle in1915, Egypt started to fight against the disease by implementing pilot projects using either snail control or chemotherapy. In Egypt, between 1989 and 1996, about 2.5 million schistosomiasis cases were diagnosed and treated, mentioning that the prevalence of schistosomiasis was close to 40% in 1983 at the national level.   The Ministry of Health and Population in Egypt has announced the start of a campaign to confirm the final elimination of schistosomiasis by 2020 after the ministry has achieved success in reducing the prevalence of schistosomiasis to about 0.2% by the end of 2016.

The-evolving-schistosomiasis-agenda-2017-2020-in-Egypt-Final-Edition-converted

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Vol.3 No.2 – 2 :Protective effect of Zamzam water against kidneys damage induced in male rats: Immunehistochemistry evidence

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By :Abbas Ch. Mraisel, Anas S.Abu ali & Inas,I.Waheeb

Abstract

Aim of study: The study was performed to investigate the role of Zamzam water (ZW) as antioxidant against histological changes that occurring in renal damage induced by n-hexane intoxication in rats by using immunohistochemical technique.

Method: The experiment was carried out at Environmental Toxicology Laboratory, Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. A total of 20 male albino rats weighing 150-170g were obtained from the animal house of the Faculty of Medicine, Alexandria University. The rats were divided to four groups (5 rats in each cage). Control group were fed basal diet and given tap water (100ml/cage) daily for ten days. In group two the rats were given (n-hexan 300uL/kg .B.W) mixed with (0.5 ml) corn oil to each rat for last five days of experimental. Group three the rats were given (100ml/cage) of Zamzam water as drinking water daily for ten days. Group four the rats were given (100ml/cage ) of Zamzam water for five days , after that given n-hexan 300uL/kg .B.W) mixed with (0.5 ml) corn oil last five days of experimental with continuous given Zamzam water . Kidney tissues of each rat were immediately removed and after weighted put into 10% neutral buffer formalin as a fixative solution. Ki-67 or P53 receptor subunits were examined in deparaffinized sections (5 µm) using an Avidin-Biotin-Peroxidase (ABC) immunohistochemical method.

The results: The results observed significantly increase in the weight of the kidneys in the group treated with n-hexane in compared with control group, also relative decrease in weight of the kidneys in the group co-treatment with zamzam water and n-hexane. The detection and distribution of PCNA immunoreactivity (PCNA-ir) in the kidney sections in the different groups under study were observed. Faint positive reaction for PCNA-ir in the kidney sections in control and Zamzam water group, Strong positive reactions for PCNA-ir were detected in n-Hexane group, while a moderate positive reaction for PCNA-ir in the kidney sections with pre -treatment Zamzam water revealed normal structure of malpighian capsule and renal tubules with moderate degeneration of epithelia cell.  Conclusions: Exposure to n- hexane showed higher toxic effect with severe kidney damage and treatment with zamzam water alone improved the antioxidant status of rats and could be useful as antioxidant against environmental stress induced by toxic chemicals.

Vol.3 No.2 - 2

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Vol3 No.2 -1 :Prevention of Etoposide induced kidney toxicity, electrolytes, injury and KI67 alternations in male rats treated with star anise

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By :Ali Salman Hassan Al-Ameri

Abstract

Star anise (Illicium verum) has been used in folk medicine of China, India, and most other Asian countries to treat stomach aches, vomiting, insomnia, dermal inflammation and rheumatic pain as well as a common spice usage. The present study was designed to investigate the possible ameliorating effect of star anise fruit extract against Etoposideinduced nephrotoxicity in rats to prove the evidence of its traditional pharmacological effectiveness. A total of 40 male Wister albino rats were divided randomly into four groups (1st group was control; 2nd group was treated with star anise, 3rd group was received Etoposide, and 4th group was treated with both star anise and Etoposide. The administration of Etoposide significantly caused elevation in creatinine, blood urea, sodium, potassium and chloride while calcium ions was significantly decrease when compared with control group. Co-treated rat with star anise and Etoposide maintained the levels of the measured some parameters (creatinine, chloride and calcium ions) closer to the normal values while urea, sodium and potassium ions were significantly decrease when compared with Etoposide group. Histopathological evidence, together with observed Ki67-ir, supported the detrimental effect of Etoposide and the ameliorating effect of star anise water extract on renal toxicity. Finally, it could be concluded that star anise has a promising role and it worth to be considered as a natural substance for ameliorating the renal toxicity and injury induced by Etoposide chemotherapy.

2017 JBSAR Ali Alameri

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