By: Anas, S. Abuali

Biology Department / Basic Education College / Missan University, Iraq

Abstract

Aim of the study: Use extraction of medical herbal such as powder ginger extract observed to stimulate the hematological system as evidenced by a decrease in the total count of RBCs, platelets as well as hemoglobin percentage. This study aimed to determine the antioxidant activity and protective effect of ginger essential oil against the toxic effect of crude oil on the hematological parameters.

Method: Crude oil was taken from the medical laboratory center in Missan province. Crude oil obtained from Missan oil company (MOC) Ltd sample type (No:38DI) Ginger (Zanjabar offcienales) was obtained from the local market, Missan city, and classified in the Department of Biology, Faculty of Science.

Experimental animals: Twenty-four Albino Waster male rats weighing (150-200 g) were assigned into three groups (8 rats for each group). Group one was administered corn oil at a dose of 1ml for each rat daily basis for 30 days, group two was treated with ginger essential oil orally (50 mg/kg B.W.) once daily for 30 days, group three was treated orally with 600ul of crude oil mixed with 1 ml corn oil orally once daily for 30 days. Blood samples were collected in clean class tubes with EDTA anticoagulant. Complete blood pictures (CBC) shown from collected blood samples by automatic method (Celltac X kx 021n automated hematology analyzer, Japan CARE Co, LTD). The results: The results observed that ginger essential oil is composed of 55 chemical components identified according to retention times and area parentage area in the extracted oil. The main active chemical components were Zingiberen in percentage (17.1), followed by Cyclohexene (α-Sesquiphellandrene) in percentage (12.1%), à-Farnesene in percentage (11.9%) and Benzene (7.9%). Red blood cells (RBCs) observed a significant decrease in the count of red blood cells (RBCs) when administrated crude oil orally as a compared control group, while the rats that were given ginger essential oil observed no significant changes in RBC count. Decrease in hemoglobin concentration (Hb), Packed cell volume (PCV), and Platelets(Plt)after administrated of crud oil in dose 600ul, while the rats that administrated GEO observed no significant changes in Hb, PCVand Plt as compared with the control group. A significant increase in White Blood Cells(WBCs), Granulocytes (Neutrophils), Monocytes, and Lymphocytes after administration of crude oil as compared with the control group, while administration of GEO orally improved activity of WBCs, Granulocytes, Monocytes, and Lymphocytes.

Conclusions: Exposure to crude oil may lead to abnormal changes in the hematological parameters and Ginger essential oil may have properties for protecting and ameliorating the toxic effects of crude oil even in high doses.

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