Posts

Vol.8 No.1 – 1: In utero exposure of green coffee extract alters rat fetal neurodevelopment in a dose dependent manner

/0 Comments/in , , /by

By: Marwa Nabil Atallaha*, Amira S. Abd El-Gaberb

a Vertebrates, Comparative Anatomy, and Embryology, Zoology Department, Faculty of Science-Menoufia University, Egypt.

b Chemistry Department, Faculty of Science, Menoufia University, Egypt

Abstract

Green coffee consumption has gained wide popularity, possibly due to its strong antioxidative activities and many beneficial effects in various human diseases. However, the effect of green coffee extract consumption on the development of the fetal central nervous system during pregnancy has not been elucidated. Consequently, the present study aimed to evaluate the effect of maternal administration of some doses of the green coffee extract on the development of the cerebral cortex, cerebellum, and spinal cord of rat fetuses in terms of histopathological, proliferation, astrogliosis, and ultrastructural investigations. Pregnant dams were divided into four groups; control group (administered distilled water) and three groups orally administered three different doses of green coffee extract, GC1 (200 mg/kg), GC2 (400 mg/kg), and GC3 (600 mg/kg) from the sixth day to the 15th day of gestation. On the 20th day, dams were sacrificed and fetal cerebral cortex, cerebellum, and spinal cord from different groups were fixed for subsequent investigations. The results showed that green coffee extract induced various histopathological changes in the three investigated organs including pyknosis, hemorrhage, and vacuolation. Immunohistochemical investigation revealed that green coffee extract decreased neuronal proliferation and increased reactive astrogliosis. Ultrastructurally, green coffee extract caused cytoplasmic rarefaction, neuronal degeneration, macrophage activation, and axon degeneration. Interestingly, the neurotoxic effects of green coffee on neuronal development were dose-dependent. Based on these results, the consumption of high doses of green coffee during pregnancy should be restricted. Moreover, further studies are needed to evaluate the long-term effects of green coffee ingestion on neuronal cognition and behavioral outcomes.

In-utero-exposure-of-green-coffee-extract-alters-rat-fetal-neurodevelopment-in-a-dose-dependent-manner-1-1-1

Download PDF

Vol.1 No.6 -3 : Comparison between the ameliorative potentials of canagliflozin and metformin on the testicular damage in diabetic rats .

/0 Comments/in , , /by

By : Margit Semmler1, Abdel-Baset M. Aref2

Abstract

To evaluate the Renoprotective efficacy of melatonin against long term exposure induced changes of dimethylbenz(a)anthracene (DMBA), cell proliferation and DNA synthesis was determined in the epithelial cells of cortical and medullary renal tubules in female and male mice applying quantitative autoradiographic analysis and using 3H thymidine as a radioactive label. A total of 30 male and female adult albino mice were divided into 3 groups, each of 10 individuals: control (group C), DMBA exposed (group D) and DMBA/melatonin exposed (group D+M) mice. In female mice, long term exposure for 150 days to a single injection of DMBA (10mg/ 100g b.w.) stimulated the incorporation rate of 3H thymidine into the epithelium of cortical renal tubules by 6774% compared to control. The number of grains over labeled nuclei was reduced by 57.1%. Daily injected with melatonin (100 g/ 100g b.w.) during the last 60 days of exposure to a single dose of DMBA attenuated cell division rate of the epithelial cells by 80% compared to group D, but remained 1275% higher than that of group C. The mean grain count over labeled nuclei was increased by 59.5% compared to group D, but remained 31.5% lower than that of control. In medullary portion of the renal tubules, DMBA induced changes were less pronounced than that in the cortical area. The cell division was stimulated by 833% compared to control and remained 8.1x lower than the percentage increase in the cortical part. The mean grain count over labeled nuclei was reduced by 40.4% compared to group C. Daily injected with melatonin (100 g/ 100g b.w.) during the last 60 days of exposure to a single dose of DMBA reduced the mitotic cell division by 83.9% compared to group D and thereby showing a similar effect as in the cortical part of the renal tubules. Compared to group C, the 3H labeling index remained 50% higher. In the cortical portion, the comparable value was 1275% higher than that of control. The mean grain count over labeled nuclei was increased by 26.2% compared to group D and remained 24.8% lower than in group C.

3. Comparison between the ameliorative potentials of canagliflozin and metformin on the testicular damage in diabetic rats .
Download Issue

Vol.1 No.6 -5 : The renoprotective efficacy of melatonin against dimethylbenz[a]anthracene induced changes after long term exposure in Mice.

/0 Comments/in , , /by

By : Margit Semmler1, Abdel-Baset M. Aref2

Abstract

To evaluate the Renoprotective efficacy of melatonin against long term exposure induced changes of dimethylbenz(a)anthracene (DMBA), cell proliferation and DNA synthesis was determined in the epithelial cells of cortical and medullary renal tubules in female and male mice applying quantitative autoradiographic analysis and using 3H thymidine as a radioactive label. A total of 30 male and female adult albino mice were divided into 3 groups, each of 10 individuals: control (group C), DMBA exposed (group D) and DMBA/melatonin exposed (group D+M) mice. In female mice, long term exposure for 150 days to a single injection of DMBA (10mg/ 100g b.w.) stimulated the incorporation rate of 3H thymidine into the epithelium of cortical renal tubules by 6774% compared to control. The number of grains over labeled nuclei was reduced by 57.1%. Daily injected with melatonin (100 g/ 100g b.w.) during the last 60 days of exposure to a single dose of DMBA attenuated cell division rate of the epithelial cells by 80% compared to group D, but remained 1275% higher than that of group C. The mean grain count over labeled nuclei was increased by 59.5% compared to group D, but remained 31.5% lower than that of control. In medullary portion of the renal tubules, DMBA induced changes were less pronounced than that in the cortical area. The cell division was stimulated by 833% compared to control and remained 8.1x lower than the percentage increase in the cortical part. The mean grain count over labeled nuclei was reduced by 40.4% compared to group C. Daily injected with melatonin (100 g/ 100g b.w.) during the last 60 days of exposure to a single dose of DMBA reduced the mitotic cell division by 83.9% compared to group D and thereby showing a similar effect as in the cortical part of the renal tubules. Compared to group C, the 3H labeling index remained 50% higher. In the cortical portion, the comparable value was 1275% higher than that of control. The mean grain count over labeled nuclei was increased by 26.2% compared to group D and remained 24.8% lower than in group C.

anthracene induced changes after long term exposure in Mice.”]
Download Issue