Vol.8 No.2 – 7:The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique

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By: Alaa Saadi Abbood

Department of Biology, Al-Farabi University College (FUC), Al-Dora Square, Al-Masafee Street, Baghdad, Iraq

Abstract

Preterm birth (PTB) and PE are the two major causes of perinatal mortality, morbidity, and long-range neurological disability. PTB defined as delivery before 37 weeks gestation, has become an epidemic in developed countries, Indeed PTB and PE are leading causes of maternal and neonatal death worldwide. The immunohistochemical study show: – The staining intensity for PE immunohistochemical showed the greatest (+2) intensity was recorded at 52% for anti-CD3 lymphocyte biomarker. Also, followed by 28% of (+1) Intensity was comparable biomarkers. (12%) of +3 and 8% for 0 intensity when compared with Tonsil control positive and placental tissue control negative. On the other hand, the staining intensity for PE immunohistochemical shows the greatest frequency of (0) intensity was recorded for anti-CD8 cytotoxic T-cell biomarker with 52 % then +1 (28%) and 20% for +2 when compared with skin control positive and negative.

The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique (1)

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Vol.8 No.2 – 6: XANTHOGRANULOMATOUS PROSTATITIS

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By: Farouk Hachem, Imad Boualaoui, Ali Mikou, Zouaidia Fouad, Ahmed Ibrahimi, Hachem Elsayegh and Yassine Nouini.

Urology “A” Department, University Hospital Center of Rabat, Morocco Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco

Abstract

Xanthogranulomatous prostatitis is a rare chronic inflammatory condition and may simulate prostatic carcinoma both clinically and microscopically. Conservative treatment is recommended for xanthogranulomatous prostatitis, including the use of alpha-blockers and corticosteroids.
Surgical options, such as transurethral prostatic resection or open adenomectomy, are reserved for patients who are severely symptomatic or who have failed conservative treatment.

XANTHOGRANULOMATOUS PROSTATITIS 2

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Vol.8 No.2 – 5: Evaluation of the diagnostic performances of tissue inhibitors of metalloproteinase-1 and fibronectin for heart failure

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By: Manar S. Fouda1, Bassem Zarif 2, Victoria Samir1, Sara A. Mekkawy3, Mohamed M. Omran1

1 Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt

2Consultant Cardiology, National Heart Institute, GOTHI, Cairo, Egypt

3 Molecular Biotechnology program, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt

Abstract

Previous research has linked an imbalance of the tissue inhibitors of metalloproteinase-1 (TIMP-1) and fibronectin (FN) to heart failure as a part of the extracellular matrix network (ECM) biochemistry profile, which is vital for cardiac homeostasis. This study aimed to assess the diagnostic performance of FN, TIMP-1, and CK-MB in heart failure (HF). Sixty patients (45 with acute and 15 with chronic HF) were recruited. Thirty individuals (20 with ischemic heart diseases, as other cardiac diseases, and 10 healthy individuals) were recruited as a control group. The biotin double antibody sandwich technology determined levels of human fibronectin and tissue inhibitors of metalloproteinase-1. FN was the most effective biomarker in differentiating HF patients from healthy individuals (AUC = 0.850) (P < 0.001), followed by TIMP (AUC = 0.74) and CK MB (AUC = 0.660). The sensitivity and specificity of FN were 82% and 70%, respectively, at a cutoff of 80 ng/ml. In addition, FN and TIMP had the same AUC (0.71) and efficiency (65%) in distinguishing HF patients from controls, followed by CK-MB (AUC = 0.70). We developed a novel model for HF diagnosis named the HFD model based on three biomarkers (FN, TIMP, and CK MB). The HFD model had an AUC of 0.77 in distinguishing HF patients from healthy individuals, with a sensitivity, specificity, and accuracy reaching 80%. For differentiating HF patients from controls, the HFD model had 0.8 AUC, 76% sensitivity, 75% specificity, and 76% accuracy.

Evaluation of the diagnostic performances of tissue inhibitors of metalloproteinase

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Vol.8 No.2 – 4: A Case Report of Lymphoepithelioma-like, a Variant of Urothelial Carcinoma of the Urinary Bladder

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By: Farouk Hachem, Hussein Abdallah, Ahmed Ibrahimi, Hachem Elsayegh and Yassine Nouini

Urology “A” Department, University Hospital Center of Rabat, Morocco

Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco

Abstract

Lymphoepithelioma-like carcinoma is an undifferentiated carcinoma with histological features similar to undifferentiated, non-keratinizing carcinoma of the nasopharynx. Lymphoepithelioma-like carcinoma of the urinary bladder is uncommon with a reported incidence of 0.4%- 1.3% of all bladder cancer. We report a case of a 72 year-old-man with a muscle-invasive lymphoepithelioma-like carcinoma of the bladder who was treated with radical cystectomy with a 12-month follow-up.

A Case Report of Lymphoepithelioma (1)

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Vol.8 No.2 – 3: Histopathological study of thyroid carcinoma in Misan province

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By: Osamah Nassir Wali1, Abbas Ch. Mraisel2, and Alaa N. Salih3          

1- Histology / Basic Medical Science Department / Nursing College / Misan University, Iraq

2- Toxopathology / Basic Medical Science Department / Nursing College / Misan University, Iraq

3- Histology /Biology Department /College of Education for Pure Sciences /Wasit University, Iraq

Abstract

Aims: “Thyroid carcinoma is specific malignant tumors derived from follicle cells including papillary and follicular carcinoma, and parafollicular, calcitonin-producing C-cells (medullary carcinoma)”. This study aimed to provide a present outlook on thyroid carcinoma among patients and investigation the relationship between the age, sex, and thyroid carcinoma in Missan province in Iraq. Methods: The data were collected in the period (First June – 2020 to Thirty November -2020) from (42) patients aged between (35 -80 or more) infected with thyroid carcinoma, a histopathological examination that performed and confirmed in the tissue sample obtained from the patients after surgical operations in AL-Sadder hospital in Missan province. Results: “The patients with thyroid carcinoma observed high incidence in ages between (41-55) years (42.8 %) cases, followed by the ages between (56-70) years (28.5 %) cases, while the lowest incidence observed in ages between (71or more) years (11.9%)cases. Thyroid carcinoma is observed more commonly found in females (64.28%) cases than the males (35.71%) cases (P < 0.05). Histopathological examination of the tissue specimens shows the commonest type of thyroid carcinoma was the papillary carcinoma” 28(66.6) cases characterized by multifocal with papillary architecture, changes in the size of thyrocytes with an abnormal nucleus and cytoplasm and shape, followed by follicular carcinomas 9(21.4) cases characterized by presence malignant epithelial cells arising from follicular cells and invasion into surrounding thyroid tissue, while the lowest type was Medullary carcinoma  5(11.9)cases, showing islands of tumor cells with central necrosis surrounded by vascular strom. Conclusion: “Papillary carcinoma is the most common malignant tumor of the thyroid gland and as compared with follicular carcinomas and medullary carcinoma. Proper implementation of preventive measures such as changing lifestyle factors might enhance control of thyroid carcinoma”.

Histopathological study of thyroid carcinoma in Misan province-converted (2)

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Vol.8 No.2 – 2: Frequency and specificity of red blood cells alloantibodies among Sudanese multiparous women

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By: Nour Mahmoud Abdelateif Ali 1, and Abather Alsayed Mohamed 2

  1. Department of Hematology, Faculty of Medical Laboratory Sciences, Alneelain University, Khartoum, Sudan
  2. Department of Hematology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Khartoum, Sudan

Abstract

Background: Allo-immunization is a condition in which the body gains immunity, from another individual of the same species, against its cells. Blood group antibodies are called alloantibodies if they react with alloantigens on the red blood cells (RBCs) of other individuals. Immune antibodies are produced following exposure to foreign erythrocyte antigens through pregnancy or transfusion. Maternal alloimmunization, also known as iso-immunization, occurs when a woman’s immune system is sensitized to foreign erythrocyte surface antigens, stimulating the production of immunoglobulin G (IgG) antibodies. Purpose: This study aimed to detect the frequency and specificity of alloantibodies against (RBCs) antigens among Sudanese multiparous women.

Methods: This cross-sectional study was conducted on randomly selected 130 pregnant ladies, most of them were attending Soba teaching hospital and the remaining to Khartoum teaching hospital during the period from February-2008 to May-2008. Serum from each lady was collected. Each sample was grouped and screened for alloantibodies, then samples with positive antibody screening were tested for antibody identification using the gel agglutination method. Results: The overall frequency of alloantibodies detected among studied pregnant ladies was 10.8%. The results also revealed that an insignificant correlation was found between age, ethnic group, and presence of alloantibodies ((P-value: > 0.05); While a significant correlation was observed between the history of abortion, and the number of pregnancies and presence of alloantibodies (P-value: < 0.05).

Conclusion: In summary, we concluded that the alloantibodies were detected in one-tenth of the studied subjects with a significant association between detected antibodies and the number of pregnancies.

Frequency and specificity of red blood cells allo-antibodies among Sudanese multiparous women-converted

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Vol.8 No.2 – 1:Induction of renal cell line apoptosis by hydrogen peroxide

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By: Khalil A. Alhalfawy1, Bahgat A. Elfiky2, Ahmed M. Zahran3 and Mahmoud M. Zayed2

  1.  Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt
  2. Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute – University of Sadat City, Egypt
  3. Internal medicine and nephrology Department, Faculty of medicine – Menoufia University, Egypt

Abstract

Background: Hydrogen peroxide is currently the most widely used as an apoptosis inducer due to its broad cytotoxic efficacy against nearly all cell types. cytotoxicity is achieved over a wide range of doses

Objective: To evaluate the cytotoxic effect of hydrogen peroxide on renal cell lines by detecting RIPK1.

 Methods: In this study, we used a Vero cell line treated with H2O2 at concentrations 0.1 mM, 0.2 mM, 0.4 mM, 0.8 mM, and 1.6 mM and examined after 30 min,1hour,2hours,3hours, 4hours and 5 hours. by using MTT assay to detect cytotoxicity to cell line (by detecting cell viability). Spectrophotometrically measure the absorbance at a wavelength of 570 nm. Measure the background absorbance of multi-well plates at 690 nm and subtract from the 450 nm measurement. Sub-lethal dose to renal cell line is one treated with 1.6 mM for 5 hours. groups group 1: renal cell line as control which not treated by H2O2. group 2: Sub-lethal which renal cell line treated by concentration 1.6 mM of H2O2 for 5 hours. Results: H2O2 is cytotoxic to renal cell line by concentration from 0.1 mM to 1.6 mM. RIPK1 gene expressed in renal cell line treated by H2O2. The sublethal dose reached 1.6 mM for 5 hours.  There is a significant difference between the 2 groups by detecting the expression of the RIPK1 gene.

Induction

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