Vol.7 No.1 – 2: Molecular detection of food fraud targeting mitochondrial 12S rRNA gene sequencing

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By : Sobhy El-Sayed Hassab El-Nabi1; Doaa Hussein2; Asmaa Galal Khallafa1

1Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Science, Menoufia University, Egypt
2Department of Zoology, Faculty of Science, Menoufia University, Egypt

Abstract

Food adulteration is a current socioeconomic crisis all over the world. Therefore, the current study aimed to use the molecular sequencing of 12S rRNA to detect food fraud in the most consumed meat cuts in the Egyptian markets. After sequences’ trimming, the fragments’ lengths were 389-395 bp for B. bubalis, B. taurusO. aries, and C. hircus. The results detected species substitution in the analyzed meat cuts. Particularly, buffalo and goat samples were replaced by cattle and buffalo. Finally, molecular methods are accurate and sensitive for the authentication of meat and are crucial in establishing the quality and authenticity of meat‐based food products.

Molecular detection of food fraud targeting mitochondrial 12S rRNA gene sequencing-converted

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Vol.7 No.1 – 1: Assessment of Safe Handling and Disposal of Laundry in Hemodialysis Unit

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By : Soheir Abouelfadle1; Thoraya Abdel Aziz  1; Doaa Amin Ahmed Sayed Ahmed2

1Medical-Surgical Nursing Department, Faculty of Nursing, Alexandria University, Egypt
2Medical-Surgical Nursing Department, Faculty of Nursing, Damanhour University, Egypt

Abstract

Handling laundry is a major task that must be managed safely by health care workers (HCW) for maintaining a green hospital environment. Laundry processing, especially in hemodialysis units is a source of many hazards, especially bacteriological ones. This study aimed to assess safe laundry handling and disposing of in hemodialysis units. A descriptive research design was utilized. Subject A convenience sample of 46 nurses (nursing and auxiliary members) was enrolled in this study. Materialsand method: The current study was conducted in dialysis units at the following Hospitals; Students’ University and Health Insurance (Gamal Abd-El-Naser); Alexandria. Tool: One tool was utilized “Safety practices of laundry handling and disposing of in hemodialysis units: Observational Checklist”; it was developed by the researchers after a thorough review of related literature. It comprised two parts, Part I: Personal data as age, gender, years of experience, employment position, educational level, and previous attendance in-service training regarding safe laundry management. Part II: Safety Laundry Handling and Disposing Observational Checklist. Result: This study reflected satisfactory safe practices level of laundry handling scores in both settings. While, no significant correlation was detected between both settings regarding safe practices of laundry handling and disposal, while a significant relationship between overall practice levels and staff socio-demographic characteristics except gender was noticed. Conclusion: A significant correlation between the availability of all-time PPE and laundry processing; with satisfactory scores for applying safety laundry practices was noticed. Recommendations: Periodic monitoring for staff as well as continuous training programs regarding safe laundry processing must take place. All linen must be handled, stored, and transported in a manner that maintains a green environment for all patients, health care workers, as well as visitors for the sustainability of health and safety in hemodialysis units.

Assessment of Safe Handling and Disposal of Laundry in Hemodialysis Unit-converted

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