Vol.7 No.1 – 4: Lactate dehydrogenase level as a COVID-19 biomarker

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By: Ahmed Abdelhalim Yameny

Society of Pathological Biochemistry and Hematology, Egypt

Email: dr.ahmedyameny@yahoo.com

DOI: 10.21608/jbaar.2021.173662

Abstract:

Background: Lactate dehydrogenase (LDH) is an intracellular enzyme found in cells in almost all organ systems, which catalyzes the interconversion of pyruvate and lactate, LDH has been proved to be a prognostic factor with

high accuracy in diseases involving multiple organ injuries such as acute heart failure (AHF) and severe acute pancreatitis (AP), LDH is a potential prognostic biomarker in patients with COVID-19. Patients and methods: This study included 217 non hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included, Assay procedure as manufactory instructions and Reagents of Chema Diagnostics Italy, LDH FL of reagent based on  DGKC recommendations. Results: The percentage of COVID-19 non hospitalized Patients relation to the serum LDH level, The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, female gender was more frequent (n=119, 54.8%) than Male gender (n=98, 45.2%). This study reveals high serum LDH levels in 147 patients (67.7%), which was a significant biomarker for COVID-19 diagnosis in non-hospitalized patients (Outpatients and patients under home observation), with a p-value is 0.024 which less than 0.05. Conclusion and Recommendations: Serum LDH level is a good biomarker of infection in COVID-19 Outpatients and patients under home observation increased by 67.7%, many other studies revealed LDH is a good marker for COVD-19 hospitalized patients and severe infection.

Lactate dehydrogenase level as a COVID-19 biomarker-converted (3

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Vol.7 No.1 – 3: Ferritin as a biomarker of infection in COVID-19 non-hospitalized patients

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By : Ahmed Abdelhalim Yameny

Society of Pathological Biochemistry and Hematology, Egypt

Abstract

Background: Serum ferritin is an iron storage protein with a primary role of regulating cellular oxygen metabolism, Ferritin has emerged as a key in the immune system, and its role as an acute phase reactant, recent studies have focused on the role of serum inflammatory markers that predict COVID-19. Patients and methods: This study included 210 non hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included, Assay procedure as manufactory instructions and Reagents of PerkinElmer Health sciences. Inc USA kit human ferritin enzyme immunoassay test catalog number 10601 was used. Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, the female gender was more frequent (n=132, 62.9%) than Male gender (n=78, 37.1%). This study reveals high serum ferritin level in 150 patients (71.4 %), which was a significant biomarker for COVID-19 diagnosis in non-hospitalized patients (Outpatients and patients under home observation), the p < /em>-value is .014738. Significant at p < /em>< .05. Conclusion and Recommendations: Serum ferritin level is a good biomarker of infection in COVID-19 non-hospitalized patients, many other studies revealed ferritin is a good marker for COVD-19 hospitalized patients,  Increased ferritin levels could cause a cytokine storm by exerting direct immunosuppressive and pro-inflammatory effects, It has been reported that fatal outcomes by COVID-19 are accompanied by cytokine storm syndrome,  Many individuals with diabetes and immunosuppressive diseases exhibit elevated serum ferritin levels, and it is known that they face a higher probability to experience serious complications from COVID-19 infection, so these patients must go to the medical laboratory for serum ferritin level analysis, to decrease ferritin levels might be the treatment with iron chelators. Deferoxamine, which was approved by the FDA, with decreasing dietary iron should be also considered as they have been shown to modify serum ferritin levels.

Ferritin as a biomarker in COVID-converted

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Vol.7 No.1 – 2: Molecular detection of food fraud targeting mitochondrial 12S rRNA gene sequencing

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By : Sobhy El-Sayed Hassab El-Nabi1; Doaa Hussein2; Asmaa Galal Khallafa1

1Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Science, Menoufia University, Egypt
2Department of Zoology, Faculty of Science, Menoufia University, Egypt

Abstract

Food adulteration is a current socioeconomic crisis all over the world. Therefore, the current study aimed to use the molecular sequencing of 12S rRNA to detect food fraud in the most consumed meat cuts in the Egyptian markets. After sequences’ trimming, the fragments’ lengths were 389-395 bp for B. bubalis, B. taurusO. aries, and C. hircus. The results detected species substitution in the analyzed meat cuts. Particularly, buffalo and goat samples were replaced by cattle and buffalo. Finally, molecular methods are accurate and sensitive for the authentication of meat and are crucial in establishing the quality and authenticity of meat‐based food products.

Molecular detection of food fraud targeting mitochondrial 12S rRNA gene sequencing-converted

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Vol.7 No.1 – 1: Assessment of Safe Handling and Disposal of Laundry in Hemodialysis Unit

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By : Soheir Abouelfadle1; Thoraya Abdel Aziz  1; Doaa Amin Ahmed Sayed Ahmed2

1Medical-Surgical Nursing Department, Faculty of Nursing, Alexandria University, Egypt
2Medical-Surgical Nursing Department, Faculty of Nursing, Damanhour University, Egypt

Abstract

Handling laundry is a major task that must be managed safely by health care workers (HCW) for maintaining a green hospital environment. Laundry processing, especially in hemodialysis units is a source of many hazards, especially bacteriological ones. This study aimed to assess safe laundry handling and disposing of in hemodialysis units. A descriptive research design was utilized. Subject A convenience sample of 46 nurses (nursing and auxiliary members) was enrolled in this study. Materialsand method: The current study was conducted in dialysis units at the following Hospitals; Students’ University and Health Insurance (Gamal Abd-El-Naser); Alexandria. Tool: One tool was utilized “Safety practices of laundry handling and disposing of in hemodialysis units: Observational Checklist”; it was developed by the researchers after a thorough review of related literature. It comprised two parts, Part I: Personal data as age, gender, years of experience, employment position, educational level, and previous attendance in-service training regarding safe laundry management. Part II: Safety Laundry Handling and Disposing Observational Checklist. Result: This study reflected satisfactory safe practices level of laundry handling scores in both settings. While, no significant correlation was detected between both settings regarding safe practices of laundry handling and disposal, while a significant relationship between overall practice levels and staff socio-demographic characteristics except gender was noticed. Conclusion: A significant correlation between the availability of all-time PPE and laundry processing; with satisfactory scores for applying safety laundry practices was noticed. Recommendations: Periodic monitoring for staff as well as continuous training programs regarding safe laundry processing must take place. All linen must be handled, stored, and transported in a manner that maintains a green environment for all patients, health care workers, as well as visitors for the sustainability of health and safety in hemodialysis units.

Assessment of Safe Handling and Disposal of Laundry in Hemodialysis Unit-converted

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