Vol.1 No.1 -2 : Ameliorative effect of aqueous extract of rosemary on cadmium – induced kidney injury in albino rats

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By : El-Morsy*, A.M., Sakr, S.A., and Bayomy, M.F

Abstract

Cadmium (Cd) is one of the heavy metals causing risks for living organisms and induced high toxicity to different biological system. Rosemary (Rosemarinus officinalis) is plant widely used in food. It has antimicrobial, antioxidant, anti-carcinogenic properties. The present work studied the effect of rosemary leaf extract on Cd induced kidney injury in rats.Cadmium chloride (30 mg/kg b.w, 5 consecutive days/week for 8 weeks) administration increased renal MDA but decreased GSH, CAT and SOD activities versus control. In parallel, serum urea and creatinine increased. Cadmium chloride caused histological alternations involved shrinkage in glomeruli, congestion in blood vessels, dilation in renal tubules and leucocytic infiltrations. Ultrastructural observations showed abnormality in nucleus, endoplasmic reticulum, brush border, proximal and distal convoluted tubules. In the contrary, administration of aqueous extract of rosemary restored these changes. The results suggested that rosemary ameliorative these changes due to its antioxidant properties by scavenging free radicals resulting from cadmium chloride.

Vol.1 No.1 -1 : Potentiation of antifibrotic activity of Atacand using grape seed extract (Gervital) in male albino rats

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By : Faten R. Abdel- Ghaffar1, Ibrahim A. El Elaimy1, Azza M. Mohamed2 and Mona EL. Ghreeb

Abstract

Carbon tetrachloride (CCl4) is a known potent hepatotoxic agent. The present investigation clarified the ameliorative effects of Atacand and Gervital on CCl hepatotoxicity. 24 male albino rats were divided into 4 groups, 6 rats each. Group I, served as normal control. Group II, animals were injected subcutaneously with CCl (1ml/kg b.w.) twice a week for 90 days. Group III, rats were injected with CCl4, as Group II, then treated orally with Atacand (8mg/kg b.w.), daily, for 30 days. rats were injected with the same dose of CCl then treated with Atacand (8mg/kg b.w.) along with Gervital (100mg/kg b.w.), orally, daily for 30 days. treatment showed a marked deterioration of liver function as a high increase in the levels of serum AST and ALT (153.8% and 157.8%, respectively). Also a significant decrease in liver antioxidant machinery was represented by reduced glutathione (GSH, 68.1%), glutathione transferase (GST, 69.9%) and glutathione reductase (GR, 65.65%). On the other side, the levels of liver protein carbonyl groups (PCO) and lipid peroxidation (LPO) were elevated 439.6% and 258.7%, respectively, compared to their control groups. These results indicated th damage as a result of CCl4-induced liver fibrosis. Both treatments with Atacand or Atacand + Gervital showed significant ameliorative effect for both liver and serum CCl4-induced alterations. The current study recorded the treatment with Gervital along with Atacand antifibrotic effectiveness than that of Atacand Gervital acted as a potentiator for antifibrotic activity of Atacand.

1. Potentiation of antifibrotic activity of Atacand using grape seed extract (Gervital) in male albino rats
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