Vol.4 No.4 – 19 : Annexin V and CXCR4 Expression in Adult Immune Thrombocytopenia. Control Randomized Study. (Annexin in ITP)

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By : Rania M fawzy1, Amal S Nasr1, Noha M. El Husseiny2*, Mona Gamil2, Heba H. El Demellawy3, Hend M Hassan1, Ibrahim A. Ibrahim 2

1 Clinical Pathology Department – Faculty of Medicine-Cairo University- Egypt.

2-Internal medicine Department – Faculty of Medicine- Cairo University– Egypt.

3  Internal Medicine Department- Faculty of Medicine-Beni-Sweif University- Egypt

*Correspondence: noha_el_husseiny77@yahoo.com

Abstract

Background: Annexin V is a cellular protein used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane.CXCR4 is expressed on cells of megakaryocytic series, especially platelets,  and plays an important role in megakaryopoiesis. Both were  used to study pathogenesis  of ITP in children. The aim of this study was to evaluate Annexin V as marker of early apoptosis and the CXCR4 expression as marker of megakaryopoiesis  in adult patients with ITP. Patients and methods: This study was conducted on 60 patients with ITP with a mean age 39.23± 12.49 and 40 healthy subjects with a mean age 36.08± 9.4 as a control group. Using the flow cytometry, we investigated apoptotic markers of platelets annexin V and megakaryopoiesis marker CXCR4  expression. Results: The percentage of the platelet with early apoptosis positive markers (Annexin -D1) were less frequent in the ITP group compared to the control group (p<0.001), the percentage of the platelet with late apoptosis positive markers (Annexin -D2), living platelet (Annexin-D3), necrotic platelet (Annexin-D4) were more frequent in the ITP group compared to the control group (p=0.62, p<0.001, p=0.016) respectively.  The percentage of the platelets with CXCR4 expression was lower in ITP patients compared to the controls (p<0.001). Conclusion: pathogenesis of ITP in adults is complicated. Platelet Annexin V together with CXCR4 expression may detect apoptosis resistance in adult patients with ITP.

Annexin V and CXCR4 Expression in Adult Immune Thrombocytopenia. Control Randomized Study.-converted

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Vol.4 No.4 – 18 : Knowledge of Adolescents Regarding the Factors Contributing to Mental Health at a Selected Nursing College in Indore

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By : Jinu K Rajan

Department of Nursing, College of Applied Medical Sciences

Majmaah University, Kingdom of Saudi Arabia

Email:jinukrajan@rediffmail.com

Abstract

Background: Mental health is simultaneous success at working, loving, creating with the capacity for nature and flexible resolution of conflicts between instincts, conscience and reality (American Psychiatric Association, 2001).Materials and Methods. The main objectives of the study were to assess the knowledge of adolescents regarding the factors contributing to mental health in a selected college and to compare the knowledge of adolescents on factors contributing to mental health with their selected demographic data. A cross sectional descriptive study was conducted on 100 college students between 17 to 21 years of age group, selected by stratified random sampling to assess their knowledge regarding factors contributing to mental health by using close ended questionnaire at Index Nursing College , Indore. Results : Observation shows that highest percentage (32%) of the college students were in the age group of 21 year and above of, majority (60%) of them were female students, almost all (90%) of them were from nuclear family, highest percentage (42%) of them were belongs to income group of Rs. 1251 and above, highest percentage (53%) of them were from urban area, majority (63%) of them were Hindus and majority (64%) of them received information from mass media. The total mean knowledge score of college students was 12.37±4.53 which is 56.23% of maximum obtainable score which revealing average knowledge, whereas highest mean score (1.42±0.62) which is 71% of maximum score on verbatim for relaxation revealing good knowledge & lowest mean score (3.63±1.91) which is 51.86% of maximum score on nutrition for mental health revealed average knowledge. Association of demographic variables with the knowledge  score shows that significant association was found between knowledge scores & age and class in which studying, whereas no significant association was found between knowledge score and sex, type of family, per month per capita income, residence, religion and source of information. Conclusion: From the findings of the present study it can be concluded that, the knowledge of students regarding factors contributing mental was good on regarding to relaxation. Over all mean, SD and mean score revealed that students having average knowledge regarding factors contributing mental health. The knowledge scores of the students when compared with selected demographic variables revealed significant association was found on age and class in which studying.

Knowledge of Adolescents Regarding the Factors Contributing to Mental Health at a Selected Nursing College in Indore-converted

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Vol.4 No.4 – 17 : Evaluation of circulating antigen in urine technique for diagnosis of Schistosoma haematobium infection

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By : Ahmed Abdelhalim Yameny

Society of Pathological Biochemistry and Hematology, Egypt

Ahmed A. Yameny (Email: dr.ahmedyameny@yahoo.com)

DOI: 10.21608/jbaar.2018.155494

Abstract

Background : Diagnosis of urinary and intestinal schistosomiasis by detecting circulating Schistosome antigens in serum and urine have been developed and assessed to dissolve problems with parasitological diagnosis in low endemic areas or for early diagnosis, this study aimed to evaluate commercially available kit, using circulating antigen in urine as alternative for microscopic testing of urine to diagnose S.haematobium infection Subjects and methods: This study is a cross-sectional study designed to evaluate circulating antigen a commercially available kit was used for determine urinary schistosomiasis  in the study population, this performed on 50 positive S.haematobium samples and 50 negative samples for individuals matching in age and sex , in addition, urine microscopic examination was done for detection of S.haematobium eggs by sedimentation centrifugation and nuclepore filteration techniques. Results :  The results showed that circulating antigen kit in urine had a sensitivity of 56% and specificity of 76%.. PPVs was 70% whereas NPVs was 63.3 %. As for diagnostic efficiency, it was 66%, where the area under the curve (AUC) was sufficient 0.63 . Conclusion and Recommendations: Years ago this commercial kit used in our study and in all medical laboratories in Egypt for detection of schistosome antigen in urine is called schistofast bilharzial antigen in urine , this study showed low sensitivity (56%) and lowe specificity (76) than expected resuls for detection of circulating antigen and they are generally more expensive than microscopic examination.

Evaluation of circulating antigen in urine technique for diagnosis of schistosoma haematobium infection-converted

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Vol.4 No.4 – 16 : Morphospecies diversity of soil invertebrates in Cultivated and Uncultivated fields

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By: Abrar Maqtan1, Hishamuddin Omar1, Muskhazli Mustafa1, Nur Ain Izzati M Zainudin1, Daljit Singh Karam2

1Department of Biology, Faculty of Science, Universiti Putra Malaysia 43400, Serdang, Selangor, Malaysia

2Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Abstract

The soil environment is teeming with living organisms full of biological life and is one of the most abundant and diverse ecosystems on earth. However, due to unsustainable agriculture practices, the fertile topsoil is often lost; the soil becomes useless for agriculture. Therefore, we cannot lose sight on the fundamental role of the soil biological entities in maintaining the sustainability of the soil, and the role of organism’s biodiversity in the soil to counter against various stress. Therefore, the main objective of this work is detecting diversity and density of soil invertebrates between cultivated field (Ladang 2) and uncultivated field (CEFS), Results showed that the highest abundance to invertebrates was recorded at CEFS 834 with 3.7 m2 density and the lowest was in Ladang 2: 225 with 1.2 m2 density, belonging to 35 different morphospecies from 4 Phylum, 4 subphyla, 6 class, 2 subclass and 19 orders.  A total of 15 morphospecies were collected in the cultivated field Ladang 2, while 25 morphospecies were collected in the uncultivated filed CEFS. Out of 25 morphospecies found in this area, 10 were exclusive to this environment. Annelida, Amphipoda, Isopoda, Collembola and Hymenoptera were the dominant taxa in CEFS, comprising: 8.15%, 10.55%, 17.98%, 14.14% and 26.97% respectively. But Acarina, Coleoptera and Insect larva 39.68%, 5.95% and 3.97% respectively were the dominated taxa in Ladang 2. The diversity was highest in the CEFS (H = 2.29) and lowest in the Ladang2 field (H = 1.74). Unfortunately, many aspects of the soil invertebrates have not been documented in Malaysia. For example, why they become dominant in certain location and what is the impact on that particular ecosystem.  This study showed that it is essential to maintain natural habitat because agricultural practices have major influence on soil invertebrate diversity and hopefully through this study able to suggest sound agriculture management to safeguard the soil ecosystems. Due to the limited information pertaining to soil invertebrates in Malaysia and limited scope of this study, it is premature to make any conclusion and therefore warrant further studies.

Morphospecies diversity of soil invertebrates in Cultivated and Uncultivated-converted

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